Multiphase electropatterning of cells and biomaterials
Multiphase electropatterning of cells and biomaterials
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DOI：
10.1039/b701306j
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发表时间：
2007-06-01
期刊：
影响因子：
6.1
通讯作者：
Bhatia, Sangeeta N.
中科院分区：
文献类型：
--
作者：
Albrecht, Dirk R.;Underhill, Gregory H.;Bhatia, Sangeeta N.
由包封在水凝胶中的细胞形成的组织在生物技术、基于细胞的测定和组织工程中有用途。我们先前已经提出了一种3D微图案化技术，该技术使用介电泳（DEP）力在水凝胶内快速定位活细胞，并且已经证明了通过控制微尺度细胞结构来调节组织功能的能力。该方法的限制是要求单一生物材料必须同时具有支持细胞存活和功能的生物学性质和允许有效介电泳图案化的材料性质。在这里，我们解决了这个问题，通过形成多相组织组成的微观组织亚单位的“局部相”的生物材料，这反过来又是由DEP力组织在一个单独的，机械支持的“散装相”的材料。我们首先定义介质电导率对DEP细胞图案化的速度和质量的影响。作为一个案例研究，我们然后生产多相组织与微尺度架构，结合联合收割机高局部水凝胶电导率，以提高敏感的肝祖细胞的存活率与低体积电导率所需的有效DEP微图案。这种方法能够扩大研究范围，研究3D细胞结构对不同细胞类型的影响，并且在未来可能会改善由各种模块化组织亚单位组装的不均匀组织的生物学功能。
Tissues formed by cells encapsulated in hydrogels have uses in biotechnology, cell-based assays, and tissue engineering. We have previously presented a 3D micropatterning technique that rapidly localizes live cells within hydrogels using dielectrophoretic (DEP) forces, and have demonstrated the ability to modulate tissue function through the control of microscale cell architecture. A limitation of this method is the requirement that a single biomaterial must simultaneously harbor biological properties that support cell survival and function and material properties that permit efficient dielectrophoretic patterning. Here, we resolve this issue by forming multiphase tissues consisting of microscale tissue sub-units in a 'local phase' biomaterial, which, in turn, are organized by DEP forces in a separate, mechanically supportive 'bulk phase' material. We first define the effects of medium conductivity on the speed and quality of DEP cell patterning. As a case study, we then produce multiphase tissues with microscale architecture that combine high local hydrogel conductivity for enhanced survival of sensitive liver progenitor cells with low bulk conductivity required for efficient DEP micropatterning. This approach enables an expanded range of studies examining the influence of 3D cellular architecture on diverse cell types, and in the future may improve the biological function of inhomogeneous tissues assembled from a variety of modular tissue sub-units.