Quantitative amplification of single-stranded DNA.

Quantitative amplification of single-stranded DNA.
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DOI:
10.1007/978-1-61779-998-3_23
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发表时间:
2012-01-01
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Lydall, David
Lydall, David
中科院分区:
其他
文献类型:
--
作者:
Holstein, Eva-Maria;Lydall, David

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单链DNA(ssDNA)中间体在DNA复制和同源重组、DNA损伤反应和DNA修复等过程中起着重要作用。使用ssDNA的定量扩增(QAOS),在复杂基因组中的各种细胞过程期间产生的ssDNA可以在许多单拷贝和重复基因座处定量。QAOS是一个有用的工具,可以深入了解涉及ssDNA的细胞过程以及蛋白质在调节ssDNA产生和对ssDNA反应中的作用。
Single-stranded DNA (ssDNA) intermediates play an important role in processes such as DNA replication and homologous recombination, DNA damage responses, and DNA repair. Using quantitative amplification of ssDNA (QAOS), ssDNA arising during various cellular processes in complex genomes can be quantified at numerous single-copy and repetitive loci. QAOS is a useful tool to gain insights into the cellular processes that involve ssDNA and the roles of proteins in regulating ssDNA production and responses to ssDNA.