Effects of ultrasonication on increased germination and improved seedling growth of aged grass seeds of tall fescue and Russian wildrye.
Effects of ultrasonication on increased germination and improved seedling growth of aged grass seeds of tall fescue and Russian wildrye.
复制标题
超声波处理对高羊茅和俄罗斯野麦老化草种子促进发芽和改善幼苗生长的影响
DOI：
10.1038/srep22403
复制
发表时间：
2016-03-01
影响因子：
4.6
通讯作者：
Gao W
中科院分区：
文献类型：
--
作者：
Liu J;Wang Q;Karagić Đ;Liu X;Cui J;Gui J;Gu M;Gao W
采用正交试验设计，研究了超声处理对老龄高羊茅（Festuca arundinacea）和俄罗斯野黑麦（Psathyrostaehys junceaNevski）种子萌发和幼苗生长的影响。多变量方差分析发现两两因子存在显著差异和耦合效应。超氧化物歧化酶（SOD）和过氧化物酶（POD）活性及丙二醛（MDA）含量均受影响。超声波处理对老化种子的发芽率（GP）和幼苗生长（GS）有积极的影响，为超声波处理在老化种子预处理中的应用提供了基础依据。经二元二次回归分析，四个超声因素的最佳条件为超声时间36.7 min，超声温度35℃，输出功率367 W，浸种时间4.1 h。超声波处理有促进幼苗生长的潜力。在自然贮藏条件下，高羊茅和俄罗斯野生黑麦种子的寿命分别约为9.5年和11.5年。讨论了提高GP和GS的生理机制。
The effects of ultrasonic treatments on the germination and seedling growth of aged tall fescue (Festuca arundinacea) and Russian wild rye (Psathyrostaehys junceaNevski) seeds were determined using orthogonal matrix experimental design with four ultrasonic factors. The multivariate analysis of variance detected significant differences and coupling effects of the pair-wise factors. The activities of Superoxide Dismutase (SOD) and Peroxidase (POD) and the Malondialdehyde (MDA) content were affected. The ultrasonic treatments had positive effects on the germination percentage (GP) of the aged seeds and the growth of the seedlings (GS) and therefore we provided a basic evidence for the application of ultrasonic treatment to pretreat aged grass seeds. For the four ultrasonic factors, the optimal conditions were a sonication time of 36.7 min, a sonication temperature of 35 °C, an output power of 367 W and a seed soaking time 4.1 h after binary quadratic regressions analyses. The ultrasonic treatment has the potential to improve seedling growth. Moreover, the longevity of the tall fescue and the Russian wild rye seeds was approximately 9.5 and 11.5 years, respectively, under natural conditions of storage. The physiological mechanisms that might contribute to the improved GP and GS were discussed.