Subgingival plaque microbiota in Saudi Arabians after use of miswak chewing stick and toothbrush
Subgingival plaque microbiota in Saudi Arabians after use of miswak chewing stick and toothbrush
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DOI：
10.1111/j.1600-051x.2004.00618.x
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发表时间：
2004-12-01
影响因子：
6.7
通讯作者：
Angmar-M책nsson, B
中科院分区：
文献类型：
--
作者：
Al-Otaibi, M;Al-Harthy, M;Angmar-M책nsson, B
背景：在许多发展中国家，咀嚼棒作为口腔卫生的传统手段被使用。它是由根，细枝和茎的萨尔瓦多persica或其他替代当地植物。目的：比较咀嚼棒和牙刷对沙乌地阿拉伯人牙龈下菌斑菌群的影响。进一步研究桃金酸提取物是否对龈下菌斑微生物有干扰作用。材料和方法：15名21-36岁的健康沙特阿拉伯男性志愿者被纳入一项单盲、随机交叉研究。参与者被教导如何正确使用每个设备。在基线、专业牙齿清洁后1周、误走或使用牙刷3周后进行牙菌斑DNA检测取样。微生物种类鉴定和定量采用棋盘法，使用全基因组，地高辛标记的DNA探针。在琼脂平板上用放线菌（Actinobacillus放线菌comitans）检测miswak周围的抑制区，并用巨噬细胞+/- miswak提取物进行生物测定，分析该细菌的白细胞毒性。结果：睡眠和刷牙对龈下菌群水平的影响相似。然而，放线菌单胞菌（A. actinomycetemcomitans）的数量明显减少
Background: The chewing stick, the miswak, is used in many developing countries as the traditional means for oral hygiene. It is prepared from the roots, twigs and stem of Salvadora persica or other alternative local plants.Objectives: To compare the effects of the chewing stick miswak (from S. persica) and toothbrush on subgingival plaque microflora among Saudi Arabian individuals. Further, to investigate whether components extracted from S. persica may interfere with the subgingival plaque micro-organisms.Material and Methods: Fifteen healthy Saudi Arabian male volunteers aged 21-36 years were included in a single-blind, randomized cross-over study. The participants were taught how to use each device properly. Plaque sampling for DNA test was performed at the baseline, 1 week after professional tooth cleaning, and after 3 weeks of either miswak or toothbrush use. Identification and quantification of microbial species were performed by the checkerboard method, using whole genomic, digoxigenin-labelled DNA probes. Inhibition zones around miswak were examined on agar plates with Actinobacillus actinomycetemcomitans and the leukotoxicity of this bacterium was analyzed in a bioassay with macrophages+/-extracts of miswak.Results: Miswak and toothbrushing had a similar influence on the levels of the subgingival microbiota. However, A. actinomycetemcomitans was significantly more reduced by miswak (p