Differential binding of c-Myc and Max to nucleosomal DNA.
Differential binding of c-Myc and Max to nucleosomal DNA.
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c-Myc 和 Max 与核小体 DNA 的差异结合。
DOI：
10.1128/mcb.14.6.4097-4107.1994
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发表时间：
1994
影响因子：
5.3
通讯作者：
Kingston,RE
中科院分区：
文献类型：
--
作者：
Wechsler,DS;Papoulas,O;Dang,CV;Kingston,RE
The ability of a transcription factor to function in vivo must be determined in part by its ability to bind to its recognition site in chromatin. We have used Max and derivatives of c-Myc to characterize the effect of changes of dimerization partner on binding to nucleosomal DNA templates. We find that homo- and heterodimeric complexes of these proteins bind to the CACGTG sequence in free DNA with similar affinities. Although Max homodimers bind to nucleosomes, truncated c-Myc homodimers do not. Surprisingly, modifying the c-Myc dimerization interface or changing its dimerization partner to Max enables nucleosomal DNA binding. Thus, changes in dimer structure or dimerization efficiency can have significant effects on nucleosome binding that are not predicted from their affinity for free DNA. We conclude that domains other than the basic region per se influence the ability of a transcription factor to bind to nucleosomal DNA and that changes of dimerization partner can directly affect the ability of a factor to occupy nucleosomal binding sites.
核小体在体外抑制 RNA 聚合酶 III 的转录起始和延伸。
影响因子：
11.4
作者：
Randall H. Morse
通讯作者：
Randall H. Morse
万能的核小体
影响因子：
64.8
作者：
K. Holde
通讯作者：
K. Holde