Glomerular Endothelial Cells Form Diaphragms during Development and Pathologic Conditions
Glomerular Endothelial Cells Form Diaphragms during Development and Pathologic Conditions
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DOI：
10.1681/asn.2007101138
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发表时间：
2008-08-01
影响因子：
13.6
通讯作者：
Sakai, Tatsuo
中科院分区：
文献类型：
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作者：
Ichimura, Koichiro;Stan, Radu V.;Sakai, Tatsuo
与大多数有窗毛细血管内皮细胞不同，成人肾小球内皮细胞（GEnC）通常被认为在其窗孔处缺乏隔膜，但这仍然存在争议。在这项研究中，形态学和免疫细胞化学分析表明，除了一小部分，成年大鼠的GEnC缺乏神经元窗，其中含有跨膜糖蛋白PV-1。相反，胚胎大鼠的GEnC表现出不规则的窗孔，并表达PV-1蛋白。窗孔隔膜的腔表面具有高密度的阴离子位点，从而补偿胚胎肾小球滤过屏障的功能不成熟。此外，在Thy-1.1肾炎的成年大鼠中，GEnC与无窗孔和PV-1表达显著增加，推测反映了损伤后肾小球毛细血管簇的恢复性重塑过程;因此，PV-1表达和无窗孔的再现可作为肾小球毛细血管重塑的标志。
Unlike most fenestrated capillary endothelial cells, adult glomerular endothelial cells (GEnC) are generally thought to lack diaphragms at their fenestrae, but this remains controversial. In this study, morphologic and immunocytochemical analyses demonstrated that, except for a small fraction, GEnC of adult rats lacked diaphragmed fenestrae, which contain the transmembrane glycoprotein PV-1. In contrast, the GEnC in embryonic rats exhibited diaphragmed fenestrae and expressed PV-1 protein. The luminal surface of the fenestral diaphragm possesses a high density of anionic sites, thereby compensating for the functional immaturity of the embryonic glomerular filtration barrier. In addition, GEnC with diaphragmed fenestrae and PV-1 expression were significantly increased in adult rats with Thy-1.1 nephritis, presumably reflecting a process of restorative remodeling of the glomerular capillary tuft after injury; therefore, the reappearance of PV-1 expression and diaphragmed fenestrae may serve as a marker of glomerular capillary remodeling.