Stitching together the Multiple Dimensions of Autophagy Using Metabolomics and Transcriptomics Reveals Impacts on Metabolism, Development, and Plant Responses to the Environment in Arabidopsis
Stitching together the Multiple Dimensions of Autophagy Using Metabolomics and Transcriptomics Reveals Impacts on Metabolism, Development, and Plant Responses to the Environment in Arabidopsis
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DOI：
10.1105/tpc.114.124677
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发表时间：
2014-05-01
期刊：
影响因子：
11.6
通讯作者：
Yoshimoto, Kohki
中科院分区：
文献类型：
--
作者：
Masclaux-Daubresse, Celine;Clement, Gilles;Yoshimoto, Kohki
自噬是植物生命过程中的一个基本过程，在免疫、衰老、营养循环和适应环境等方面发挥着关键作用。自噬突变体(ATG)莲座叶的转录和代谢组学研究表明，自噬对细胞的稳态和胁迫反应是必不可少的，并且影响了几条代谢途径。己糖、quercetins和花青素的缺乏与几种氨基酸和相关化合物的过度积累相平行，如谷氨酸、蛋氨酸、谷胱甘肽、哌替酸和2-氨基己二酸酯。转录数据显示谷胱甘肽、蛋氨酸、棉子糖、半乳糖醛酸和花青素的途径受到干扰。ATG突变体中的花青素耗竭是由于编码参与类黄酮生物合成的酶和调节蛋白的主要基因下调所致。此前报道的花青素耗竭可能是类黄酮运输到液泡的缺陷。过表达花青素PIGMENT1转录因子可以恢复ATG突变体液泡中花青素的积累。转录组分析揭示了自噬与(1)水杨酸的生物合成和反应，(2)细胞分裂素的感知，(3)氧化胁迫和植物防御，以及自噬和COP9信号体机制之间可能的相互作用之间的联系。对自噬突变体的代谢和转录特征进行了讨论，并显示出与观察到的表型一致。
Autophagy is a fundamental process in the plant life story, playing a key role in immunity, senescence, nutrient recycling, and adaptation to the environment. Transcriptomics and metabolomics of the rosette leaves of Arabidopsis thaliana autophagy mutants (atg) show that autophagy is essential for cell homeostasis and stress responses and that several metabolic pathways are affected. Depletion of hexoses, quercetins, and anthocyanins parallel the overaccumulation of several amino acids and related compounds, such as glutamate, methionine, glutathione, pipecolate, and 2-aminoadipate. Transcriptomic data show that the pathways for glutathione, methionine, raffinose, galacturonate, and anthocyanin are perturbed. Anthocyanin depletion in atg mutants, which was previously reported as a possible defect in flavonoid trafficking to the vacuole, appears due to the downregulation of the master genes encoding the enzymes and regulatory proteins involved in flavonoid biosynthesis. Overexpression of the PRODUCTION OF ANTHOCYANIN PIGMENT1 transcription factor restores anthocyanin accumulation in vacuoles of atg mutants. Transcriptome analyses reveal connections between autophagy and (1) salicylic acid biosynthesis and response, (2) cytokinin perception, (3) oxidative stress and plant defense, and possible interactions between autophagy and the COP9 signalosome machinery. The metabolic and transcriptomic signatures identified for the autophagy mutants are discussed and show consistencies with the observed phenotypes.