Localization of myosin II regulatory light chain in the cerebral vasculature
Localization of myosin II regulatory light chain in the cerebral vasculature
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DOI：
10.1016/j.acthis.2007.08.005
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发表时间：
2008-01-01
影响因子：
2.5
通讯作者：
Kioussi, Chrissa
中科院分区：
文献类型：
--
作者：
Ishmael, Jane E.;Lohr, Christiane V.;Kioussi, Chrissa
大脑的细胞骨架。微血管内皮细胞。细胞是血脑屏障(BBB)功能的关键决定因素。屏障完整性似乎对肌球蛋白调节紧链(RLC)中特定残基的磷酸化状态特别敏感，RLC是肌球蛋白II发动机复合体的两个附属紧链之一。肌球蛋白致密链激酶(MLCK)对肌球蛋白RLC的磷酸化与酒精滥用和低氧相关的血脑屏障功能障碍有关，而去磷酸化可能会增强降脂他汀类药物后的血脑屏障完整性。利用免疫组织化学方法，我们提供了肌球蛋白II RLC广泛分布于小鼠脑血管系统的证据。光镜下可见肌球蛋白II RLC蛋白免疫定位于脑毛细血管内皮细胞。小动脉和小静脉的细胞层。肌球蛋白RLC在非肌肉内皮细胞中的免疫标记可与较大肌小动脉中膜平滑肌层的肌球蛋白RLC免疫反应区别开来。这些发现支持将肌球蛋白II RLC作为大脑肌动蛋白细胞骨架的一个新的组成部分。内皮细胞有可能在体内对大脑的选择性脆弱性做出贡献。(C)2007年爱思唯尔股份有限公司。版权所有。
The cytoskeleton of cerebral. microvascular endothelial. cells is a critical determinant of blood-brain barrier (BBB) function. Barrier integrity appears to be particularly sensitive to the phosphorylation state of specific residues within myosin regulatory tight chain (RLC), one of two accessory tight chains of the myosin II motor complex. Phosphorylation of myosin RLC by myosin tight chain kinase (MLCK) has been implicated in BBB dysfunction associated with alcohol abuse and hypoxia, whereas dephosphorylation may enhance BBB integrity following exposure to lipid-lowering statin drugs. Using immunohistochemistry we provide evidence of widespread myosin II RLC distribution throughout the cerebral vasculature of the mouse. Light microscopy revealed immunolocalization of myosin II RLC protein in the endothelium of brain capillaries, the endothelial. cell layer of arterioles and in association with venules. Immunolabeling of myosin RLC in non-muscle endothelial cells could be distinguished from myosin RLC immunoreactivity associated with the smooth muscle layer of the tunica media in larger muscular arterioles. These findings support an emerging rote for myosin II RLC as a component of the actomyosin cytoskeleton of cerebral. endothelial cells with the potential to contribute to the selective vulnerability of the brain in vivo. (C) 2007 Elsevier GmbH. All rights reserved.