Prokka: rapid prokaryotic genome annotation
Prokka: rapid prokaryotic genome annotation
复制标题
DOI：
10.1093/bioinformatics/btu153
复制
发表时间：
2014-07-15
期刊：
影响因子：
5.8
通讯作者：
Seemann, Torsten
中科院分区：
文献类型：
--
作者：
Seemann, Torsten
.总结：当今DNA测序仪器的多重能力和高产量使得细菌全基因组测序成为常规事务。随后将读段重新组装成重叠群已经得到很好的解决。在这些重叠群上注释所有相关基因组特征的最后一步可以使用现有的基于网络和电子邮件的系统缓慢实现，但这些不适用于敏感数据或集成到计算管道中。在这里，我们介绍Prokka，一个命令行软件工具，在典型的台式计算机上大约10分钟内完全注释细菌基因组草案。它生成符合标准的输出文件，用于进一步分析或在基因组浏览器中查看。
.Summary: The multiplex capability and high yield of current day DNA-sequencing instruments has made bacterial whole genome sequencing a routine affair. The subsequent de novo assembly of reads into contigs has been well addressed. The final step of annotating all relevant genomic features on those contigs can be achieved slowly using existing web- and email-based systems, but these are not applicable for sensitive data or integrating into computational pipelines. Here we introduce Prokka, a command line software tool to fully annotate a draft bacterial genome in about 10 min on a typical desktop computer. It produces standards-compliant output files for further analysis or viewing in genome browsers.