T-CELL PROLIFERATION INDUCED BY BORRELIA-BURGDORFERI IN PATIENTS WITH LYME BORRELIOSIS - AUTOLOGOUS SERUM REQUIRED FOR OPTIMUM STIMULATION
T-CELL PROLIFERATION INDUCED BY BORRELIA-BURGDORFERI IN PATIENTS WITH LYME BORRELIOSIS - AUTOLOGOUS SERUM REQUIRED FOR OPTIMUM STIMULATION
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DOI：
10.1002/art.1780340404
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发表时间：
1991-04-01
影响因子：
--
通讯作者：
BURMESTER, GR
中科院分区：
文献类型：
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作者：
KRAUSE, A;BRADE, V;BURMESTER, GR
在24例血清阳性和血清阴性的莱姆病患者，30例不同来源的关节炎（非莱姆病关节炎），和20名正常献血员的细胞免疫反应，以伯氏疏螺旋体的研究。到目前为止，最强的T细胞刺激是通过与自体血清孵育诱导的;在与同种异体或异源血清孵育后，反应显著较低或无反应。在莱姆病患者中，包括血清阴性患者，与正常供体（平均19，700 dpm; P <0. 0001）相比，尤其是非莱姆病关节炎患者（平均11，600 dpm; P <0. 0001），对整个B伯氏菌的增殖反应显著升高（平均64，750 dpm）。莱姆病患者在成功的抗生素治疗后，增殖水平显着下降。使用B burgdorferi和噬菌体密螺旋体作为抗原的平行培养显示，莱姆疏螺旋体病患者的细胞对B burgdorferi的反应显著高于对噬菌体密螺旋体的反应，但非莱姆关节炎患者的细胞没有发生这种情况。疾病分期和增殖值之间没有相关性。这些数据表明，淋巴细胞增殖试验可能提供了一个重要的工具，莱姆病的诊断，最显着的关节炎患者和血清阴性。相反，缺乏反应性似乎是没有活动性莱姆病的强烈指标。然而，在这些测定中使用自体血清似乎是至关重要的。
The cellular immune response to Borrelia burgdorferi was studied in 24 patients with seropositive and seronegative Lyme borreliosis, 30 patients with arthritides of different origin (non-Lyme arthritides), and 20 normal blood donors. By far, the strongest T cell stimulation was induced by incubation with autologous serum; there was a significantly lower response or no response after incubation with allogeneic or heterologous sera. In patients with Lyme borreliosis, including seronegative patients, there was a strinkingly elevated proliferation in response to whole B burgdorferi bacteria (mean 64,750 dpm) compared with that of normal donors (mean 19,700 dpm; P < 0.0001) and especially that of non-Lyme arthritis patients (mean 11,600 dpm; P < 0.0001). Levels of proliferation declined significantly in patients with Lyme borreliosis after successful antibiotic treatment. Parallel cultures using B burgdorferi and Treponema phagedenis as antigens showed that cells from patients with Lyme borreliosis responded significantly more to B burgdorferi than to T phagedenis, but this did not occur with cells from individuals with non-Lyme arthritides. There was no correlation between disease stages and proliferation values. These data indicate that lymphocyte proliferation assays may provide an important tool for the diagnosis of Lyme borreliosis, most notably in patients with arthritides and in those who are seronegative. Conversely, the lack of reactivity appears to be a strong indicator of the absence of active Lyme disease. It seems to be crucial, however, to use autologous sera in these assays.