Micropipette suction for measuring piconewton forces of adhesion and tether formation from neutrophil membranes
Micropipette suction for measuring piconewton forces of adhesion and tether formation from neutrophil membranes
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DOI：
10.1016/s0006-3495(96)79486-9
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发表时间：
1996-11-01
影响因子：
3.4
通讯作者：
Hochmuth, RM
中科院分区：
文献类型：
--
作者：
Shao, JY;Hochmuth, RM
本文提出了一种利用微吸管吸力测量微牛顿尺度力的新方法。球形细胞或珠子直接用作力传感器，并且可以施加小至10-20 pN的力。当换能器在移液管中静止时，力简单地是抽吸压力和移液管的横截面积的乘积减去对换能器和移液管壁之间存在的窄间隙的小的校正。当传感器沿着移液器移动时，其上的力由与其速度相对于其无阻力速度的比率成比例的因子引导。利用这种技术，确定了从中性粒细胞形成膜系链所需的最小力（45 pN），并推断了中性粒细胞表面上微绒毛的长度。该技术的优点在于其简单性和测量细胞之间的力的能力，而不需要单独的理论或针对外部标准的校准，并且不需要使用固体表面。
A new method for measuring piconewton-scale forces that employs micropipette suction is presented here. Spherical cells or beads are used directly as force transducers, and forces as small as 10-20 pN can he imposed. When the transducer is stationary in the pipette, the force is simply the product of the suction pressure and the cross-sectional area of the pipette minus a small correction for the narrow gap that exists between the transducer and the pipette wall. When the transducer is moving along the pipette, the force on it is directed by a factor that is proportional to the ratio of its velocity relative tb its drag-free velocity. With this technique the minimum force required to form a membrane tether from neutrophils is determined (45 pN), and the length of the microvilli on the surface of neutrophils is inferred. The strength of this technique is in its simplicity and its ability to measure forces between cells without requiring a separate theory Or a calibration against an external standard and without requiring the use of a solid surface.