Dielectrophoresis of DNA: Time- and frequency-dependent collections on microelectrodes
Dielectrophoresis of DNA: Time- and frequency-dependent collections on microelectrodes
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DOI：
10.1109/tnb.2005.864012
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发表时间：
2006-03-01
影响因子：
3.9
通讯作者：
Morgan, H
中科院分区：
文献类型：
--
作者：
Bakewell, DJ;Morgan, H
本文报道了测量的特点收集的DNA到交叉指型微电极的高频介电电泳。测量的时间依赖性收集的12个酶对质粒DNA到微电极上的介电电泳显示显着减少的响应频率从100 kHz增加到20 MHz。收集时间曲线定量测量使用荧光显微镜在100 kHz至5 MHz的范围内，并表示在两个参数：初始介电电泳收集速率，和初始到稳态收集过渡。这两个参数的测量值与使用介电光谱法测量的相同质粒DNA的有效极化率的频率依赖性真实的部分的趋势一致。实验测得的参数进行定性比较，考虑到介电泳粒子的运动和扩散的理论预测的趋势。讨论了实验和理论之间的差异，并提出了对理论模型的改进建议，例如，包括电液动力驱动流体运动的影响。
This paper reports measurements that characterize the collection of DNA onto interdigitated microelectrodes by high-frequency dielectrophoresis. Measurements of time-dependent collection of 12 kilobase pair plasmid DNA onto microelectrodes by dielectrophoresis show significant reduction in the response as the frequency increases from 100 kHz to 20 MHz. Collection time profiles are quantitatively measured using fluorescence microscopy over the range 100 kHz to 5 MHz and are represented in terms of two parameters: the initial dielectrophoretic collection rate, and the initial to steady-state collection transition. Measured values for both parameters are consistent with trends in the frequency-dependent real part of the effective polarizability measured for the same plasmid DNA using dielectric spectroscopy. The experimentally measured parameters are qualitatively compared with trends predicted by theory that takes into account dielectrophoretic particle movement and diffusion. The differences between experiment and theory are discussed with suggested improvements to theoretical models, for example, including the effects of electrohydrodynamically driven fluid motion.