The effect of low-dose irradiation on unstimulated and PHA-stimulated human lymphocyte subsets.

The effect of low-dose irradiation on unstimulated and PHA-stimulated human lymphocyte subsets.
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低剂量照射对未刺激和 PHA 刺激的人类淋巴细胞亚群的影响。

DOI:
10.1080/09553008814550441
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发表时间:
1988
期刊:
International journal of radiation biology and related studies in physics, chemistry, and medicine
影响因子:
--
通讯作者:
Habbersett,RC
Habbersett,RC
中科院分区:
--
文献类型:
--
作者:
Stewart,CC;Stevenson,AP;Habbersett,RC

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使用培养系统评估从正常成年男性获得的CD 4+ 和CD 8+ T 细胞、Leu 19+ 细胞和B 细胞的放射敏感性。未刺激的 CD 8+ 淋巴细胞 (D0= 55 cGy) 的放射敏感性是 CD 4+ 细胞 (D0= 115 cGy) 的两倍。 B 细胞具有中等放射敏感性(D0= 100 cGy)。 Leu 19+ 细胞的放射抗性更强,表达的 D0 为 550 cGy。当在植物血凝素 (PHA) 刺激前 1 或 4 天照射淋巴细胞时,它们比先用 PHA 刺激然后再照射的情况对放射更敏感。当PHA刺激后1小时照射淋巴细胞时,每个淋巴细胞亚群的特征在于B细胞的D0增加到150cGy,CD4+细胞增加到290cGy,CD8+细胞增加到240cGy。相反,Leu 19+细胞在受到PHA刺激后表现出D0降低至290 cGy。
A culture system was used to evaluate the radiosensitivity of CD 4+and CD 8+T cells, Leu 19+cells, and B cells obtained from normal adult males. Unstimulated CD 8+lymphocytes (D0= 55 cGy) were twice as radiosensitive as CD 4+cells (D0= 115 cGy). B cells had an intermediate radiosensitivity (D0= 100 cGy). Leu 19+cells were much more radioresistant and expressed aD0of 550 cGy. When lymphoid cells were irradiated 1 or 4 days before phytohemagglutinin (PHA) stimulation, they were more radiosensitive than if they were first stimulated with PHA and then irradiated. When lymphoid cells were irradiated 1 h after PHA stimulation each lymphocyte subset was characterized by an increase in theD0to 150 cGy for B cells to 290 cGy for CD4+cells, and to 240 cGy for CD8+cells. In contrast, Leu 19+cells exhibited a decrease in theirD0to 290 cGy after they were stimulated by PHA.
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