Divalent metal transporter, DMT1: A novel MRI reporter protein
Divalent metal transporter, DMT1: A novel MRI reporter protein
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DOI：
10.1002/mrm.24509
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发表时间：
2013-09-01
影响因子：
3.3
通讯作者：
Turnbull, Daniel H.
中科院分区：
文献类型：
--
作者：
Bartelle, Benjamin B.;Szulc, Kamila U.;Turnbull, Daniel H.
锰（Mn）增强MRI（MEMRI）在小动物的解剖和功能成像中的应用越来越多，其基于脑、心脏和其他器官中Mn离子的细胞摄取。以前的研究依赖于顺磁性Mn离子的吸收和增强的内源性机制。为了从基因上控制MEMRI信号，我们反向工程了参与锰吸收的分子机制的主要组成部分，即二价金属转运蛋白DMT 1。DMT 1以高度敏感和动态的方式提供阳性细胞增强，与先前提出的MRI报告基因（如铁蛋白）相比，允许MRI具有更高的空间和时间分辨率。我们的特点是MEMRI信号增强特性的DMT 1表达细胞，在体外和体内的小鼠模型的癌症和脑发育。我们的研究结果表明，DMT 1提供了一个有效的遗传MRI报告广泛的生物和临床前成像应用。Magn Reson Med 70：842-850，2013。(c)2012 Wiley Periodicals，Inc.
Manganese (Mn)-enhanced MRI (MEMRI) has found a growing number of applications in anatomical and functional imaging in small animals, based on the cellular uptake of Mn ions in the brain, heart, and other organs. Previous studies have relied on endogenous mechanisms of paramagnetic Mn ion uptake and enhancement. To genetically control MEMRI signals, we reverse engineered a major component of the molecular machinery involved in Mn uptake, the divalent metal transporter, DMT1. DMT1 provides positive cellular enhancement in a manner that is highly sensitive and dynamic, allowing greater spatial and temporal resolution for MRI compared to previously proposed MRI reporters such as ferritin. We characterized the MEMRI signal enhancement properties of DMT1-expressing cells, both in vitro and in vivo in mouse models of cancer and brain development. Our results show that DMT1 provides an effective genetic MRI reporter for a wide range of biological and preclinical imaging applications. Magn Reson Med 70:842-850, 2013. (c) 2012 Wiley Periodicals, Inc.