LONGEVITY, BODY-WEIGHT, AND NEOPLASIA IN AD LIBITUM-FED AND DIET-RESTRICTED C57BL6 MICE FED NIH-31 OPEN FORMULA DIET
LONGEVITY, BODY-WEIGHT, AND NEOPLASIA IN AD LIBITUM-FED AND DIET-RESTRICTED C57BL6 MICE FED NIH-31 OPEN FORMULA DIET
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DOI：
10.1177/019262339502300503
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发表时间：
1995-09-01
影响因子：
1.5
通讯作者：
TURTURRO, A
中科院分区：
文献类型：
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作者：
BLACKWELL, BN;BUCCI, TJ;TURTURRO, A
将每种性别的C57 BL 6小鼠分组至2种饮食方案（随意（AL）或饮食限制（DR））中的一种，以研究食物限制对体重、存活率和肿瘤形成的影响。AL组和DR组再分为计划处死组（每6个月检查一次）和寿命组（提供寿命数据）。对911只动物进行尸检和显微镜检查。在终生组中，AL雄性和AL雌性的食物消耗量平均分别为33.6和34.4 g/周; DR对应组的食物消耗量减少40%。AL雄性动物、AL雌性动物、DR雄性动物和DR雌性动物的平均终生体重分别为34.8、26.8、22.6和21.6 g，50%存活时的年龄分别为27.5、26.9、31.7和33.5个月。DR男性和女性的最大寿命增加了18%。AL雄性和雌性荷瘤小鼠的终生发病率分别为89%和86%，而DR小鼠的每种性别均为64%。雌性DR小鼠的淋巴瘤（9% vs 29%）、垂体肿瘤（1% vs 37%）和甲状腺肿瘤（0.4% vs 8%）显著减少。在男性中，DR使肝细胞肿瘤从10%降低至1%。相反，DR女性中组织细胞肉瘤的发病率增加，而DR男性中未受影响。DR动物的肿瘤发病延迟;到24个月时，AL小鼠中87%的雄性肿瘤和95%的雌性肿瘤发生，而DR动物在该年龄时的发病率分别仅为其一生的52%和39%。本研究提供了以6个月间隔随机检查的C57 BL 6小鼠（横断面组）的AL和DR比较数据，并与未采样并允许自然死亡的类似队列动物（长寿组）的数据平行。饮食限制降低了荷瘤动物的寿命百分比和每只动物的肿瘤数量，并推迟了大多数肿瘤的发病年龄。
Groups of C57BL6 mice of each sex were assigned to one of 2 dietary regimens, ad libitum (AL) or dietary restriction (DR), to study effects of food restriction on body weight, survival, and neoplasia. The AL and DR groups were subdivided into a scheduled sacrifice group for examination at 6-mo intervals, and a lifetime group to provide longevity data. Necropsies and microscopic examinations were conducted on 911 animals. In the lifetime group food consumption averaged 33.6 and 34.4 g per week by AL males and AL females, respectively; the DR counterparts were given 40% less. The diet contained 4.35 kcal/g. The average lifetime body weights were 34.8, 26.8, 22.6, and 21.6 g for AL males, AL females, DR males, and DR females, respectively, and their age at 50% survival was 27.5, 26.9, 31.7, and 33.5 mo. Maximal lifespan was increased 18% in DR males and females. Lifetime incidence of tumor-bearing mice was 89% and 86% for AL males and females, versus 64% for each sex of DR mice. Dramatic reduction occurred in female DR mice in lymphoma (9% vs 29%), pituitary neoplasms (1% vs 37%), and thyroid neoplasms (0.4% vs 8%). In males, hepatocellular tumors were reduced to 1% from 10% by DR. In contrast, the incidence of histiocytic sarcoma was increased in DR females and unaffected in DR males. Tumor onset was delayed in DR animals; 87% of all neoplasms in males and 95% in females had occurred in the AL mice by 24 mo, whereas the DR animals had only 52% and 39% of their lifetime incidence, respectively, by that age. This study provided comparative AL and DR data from C57BL6 mice examined randomly at 6-mo intervals (cross-sectional group) in parallel with data from animals in similar cohort that was unsampled and allowed to succumb naturally (longevity group). Dietary restriction reduced the lifetime percentage of tumor-bearing animals and the number of tumors per animal, and delayed the age at onset of most neoplasms.