Increased nitric oxide-mediated vasodilation of bone resistance arteries is associated with increased trabecular bone volume after endurance training in rats
Increased nitric oxide-mediated vasodilation of bone resistance arteries is associated with increased trabecular bone volume after endurance training in rats
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DOI：
10.1016/j.bone.2009.10.029
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发表时间：
2010-03-01
期刊：
影响因子：
4.1
通讯作者：
Delp, Michael D.
中科院分区：
文献类型：
--
作者：
Dominguez, James M., II;Prisby, Rhonda D.;Delp, Michael D.
老年相关的骨质疏松症与骨血流量减少和一氧化氮（NO）介导的骨血管舒张功能受损有关。耐力运动训练可以恢复许多血管床中与年龄相关的血管舒张减少，并改善骨骼特性。本研究的目的是确定在所有耐力训练干预后，骨脉管系统的功能改善是否与骨特性的增加相关。年轻成年（4-6个月）和老年（24-26个月）雄性Fischer-344大鼠保持久坐或接受训练（15 m/min步行。150倾斜度，5天/周，10-12周）。使用乙酰胆碱 (ACh) 以及一氧化氮合酶 (NOS) 和环氧合酶 (COX) 抑制剂体外评估股骨主营养动脉 (PNA) 的内皮依赖性血管舒张。训练后，年轻动物的 PNA 内皮依赖性血管舒张作用增强了 16%，年老动物增强了 24%。对老年大鼠进行训练后，NOS 介导的内皮依赖性血管舒张作用增强了 77%。久坐动物的股骨远端骨小梁体积 (BV/TV, %) 随着年龄的增长而降低（年轻：27 +/- 2%，老年：23 +/- 1%；P
Old age-associated osteoporosis is related to diminished bone blood flow and impaired nitric oxide (NO)mediated vasodilation of the bone vasculature. Endurance exercise training restores the age-associated reduction of vasodilation in numerous vascular beds, as well as improving bone properties. The purpose of this study was to determine whether functional improvements in the bone vasculature are associated with increased bone properties after all endurance training intervention. Young adult (4-6 months) and old (24-26 months) male Fischer-344 rats remained sedentary or were trained (15 m/min walking. 150 incline, 5 days/week, 10-12 weeks). Endothelium-dependent vasodilation of the femoral principal nutrient artery (PNA) was assessed in vitro using acetylcholine (ACh) and inhibitors of NO synthase (NOS) and cyclooxygenase (COX). PNA endothelium-dependent vasodilation was greater after training by 16% in young and by 24% in old animals. The NOS-mediated contribution to endothelium-dependent vasodilation was enhanced by 77% after training in old rats. Distal femur trabecular bone volume (BV/TV, %) was lower with old age in sedentary animals (young: 27 +/- 2%, old: 23 +/- 1%; P