Age-specific differences in binding of heparin to plasma proteins
Age-specific differences in binding of heparin to plasma proteins
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DOI：
10.1111/j.1538-7836.2010.03847.x
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发表时间：
2010-06-01
影响因子：
10.4
通讯作者：
Monagle, P.
中科院分区：
文献类型：
--
作者：
Ignjatovic, V.;Straka, E.;Monagle, P.
工作背景：先前已在体外以及体内不同临床环境中确定了肝素抗凝作用的临床显著年龄相关差异。假设这些差异是由于肝素与血浆蛋白结合的年龄特异性差异所致。目的：本项目的目的是研究肝素与血浆蛋白结合的全球年龄相关差异。患者/方法：肝素结合蛋白通过将肝素包被的磁珠与来自新生儿、儿童和成人的血浆样品一起孵育，并纯化在该反应系统中结合至磁珠的蛋白质来鉴定。结果如下：这些结果提供了与年龄相关的肝素结合蛋白质的总数和浓度差异的第一个初步证据。结果还表明，第一次，有肝素结合抗凝血酶和凝血酶的年龄相关的差异。结论：本研究的结果虽然是初步的，但支持并有助于解释先前在体外和体内观察到的肝素效应中年龄相关差异的机制。
Background: Clinically significant age-related differences in the anticoagulation effect of heparin have previously been established in vitro as well as in different clinical settings in vivo. These differences were hypothesized to be due to the age-specific differences in binding of heparin to plasma proteins. Objectives: The aim of this project was to investigate global age-related differences in heparin binding to plasma proteins. Patients/Methods: Heparin-binding proteins were identified by incubating heparin-coated magnetic beads with plasma samples from neonates, children and adults, and purifying the proteins that were bound to the beads in this reaction system. Results: These results provide the first preliminary evidence of age-related differences in the total number and concentration of proteins bound to heparin. The results also suggest, for the first time, that there are age-related differences of heparin binding to antithrombin and thrombin. Conclusions: The results of this study, although preliminary, support and contribute to the explanation of the mechanism of age-related differences in the effect of heparin observed previously in vitro and in vivo.