Temperature-dependent biosynthesis of glucose monomycolate and its recognition by CD1-restricted T cells
Temperature-dependent biosynthesis of glucose monomycolate and its recognition by CD1-restricted T cells
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DOI：
10.1016/j.bbrc.2005.09.070
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发表时间：
2005-11-18
影响因子：
3.1
通讯作者：
Yano, I
中科院分区：
文献类型：
--
作者：
Enomoto, Y;Sugita, M;Yano, I
分枝菌酸是构成分枝杆菌富含脂质的细胞壁框架的长链脂肪酸。感染后，分枝杆菌开始利用宿主来源的葡萄糖作为糖源合成葡萄糖单分枝菌酸 (GMM)，这是一种葡萄糖基化分枝菌酸。因此，GMM 的产生是分枝杆菌局部侵袭的良好指标，宿主免疫系统对其进行检测将有利于分枝杆菌感染的有效监测。在这里，我们发现 GMM 在 30 摄氏度而不是 37 摄氏度下大量产生，并被从分枝杆菌感染的人类皮肤中分离出来的 GMM 特异性、CD1 限制性 T 细胞系识别。由于分枝杆菌感染的常见门户网站包括肺泡和外部暴露的皮肤，这些部位通常使入侵的微生物在较低的温度下生存，因此通过 CD1 脂质抗原呈递分子对 GMM 进行采样可能使宿主能够在早期阶段检测到分枝杆菌感染。 (c) 2005 Elsevier Inc. 保留所有权利。
Mycolic acids are long chain fatty acids that constitute the lipid-rich cell wall framework of mycobacteria. Upon infection, mycobacteria begin to synthesize glucose monomycolate (GMM), a glucosylated species of mycolic acids, by utilizing host-derived glucose as sugar source. Accordingly, GMM production-serves as a good indicator for local invasion of mycobacteria, and its detection by the host immune system would favor efficient monitoring of mycobacterial infection. Here, we found that GMM was produced abundantly at 30 degrees C rather than at 37 degrees C and recognized by a GMM-specific, CD1-restricted T cell line that was isolated from mycobacteria-infected human skin. Since the common portal sites for mycobacterial infection include ventilating alveoli of the lung and the externally exposed skin that often render invading microbes survive at reduced temperature, sampling GMM by CD1 lipid antigen-presenting molecules may allow the host to detect mycobacterial infection at its early phases. (c) 2005 Elsevier Inc. All rights reserved.