Serum heat inactivation affects protein corona composition and nanoparticle uptake
Serum heat inactivation affects protein corona composition and nanoparticle uptake
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DOI：
10.1016/j.biomaterials.2010.09.049
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发表时间：
2010-12-01
期刊：
影响因子：
14
通讯作者：
Dawson, Kenneth A.
中科院分区：
文献类型：
--
作者：
Lesniak, Anna;Campbell, Abigail;Dawson, Kenneth A.
纳米颗粒的大小适合与细胞相互作用，并且可能使用一系列细胞机制来内化和运输到各种亚细胞区室。现在了解到，一旦与生物流体接触，纳米颗粒表面就会被一层高度特异性的蛋白质覆盖，形成纳米颗粒蛋白质冠，这种蛋白质层的稳定时间是纳米颗粒进口的典型时间尺度的几倍。在这项工作中，通过研究血清热失活和补体耗竭对细胞内积累的纳米颗粒负荷的影响，研究了电晕成分对纳米颗粒摄取的影响。对于相同的材料和纳米颗粒大小，当纳米颗粒分散在血清热失活或不加热的介质中时，细胞摄取的显著不同即使在非特化细胞中也失活，这表明不同的血清可以导致不同的纳米颗粒剂量。摄取与结合到纳米颗粒冠上的蛋白质数量相关的事实表明，需要一个普遍同意的体外纳米颗粒细胞研究分散方案(C) 2010 Elsevier Ltd版权所有
Nanoparticles are of an appropriate size to interact with cells and are likely to use a range of cellular machinery for internalisation and trafficking to various sub-cellular compartments It is now understood that once in contact with biological fluids the nanoparticle surface gets covered by a highly specific layer of proteins forming the nanoparticle protein corona This protein layer is stable for times longer than the typical time scale of nanoparticle import, and thus can impact on particle uptake and trafficking inside the cells In this work the effect of the corona composition on nanoparticle uptake has been investigated by studying the impact of serum heat inactivation and complement depletion on the load of nanoparticles accumulated inside the cell For the same material and nanoparticle size cellular uptake was found to be significantly different when the nanoparticles were dispersed in medium where the serum was heat inactivated or not heat inactivated even for non-specialized cells suggesting that different sera can lead to different nanoparticle doses The fact that uptake was correlated with the amount of protein bound into the nanoparticle corona suggests the need for commonly agreed dispersion protocols for in vitro nanoparticle cell studies (C) 2010 Elsevier Ltd All rights reserved