Transient Protein Expression by Agroinfiltration in Lettuce
Transient Protein Expression by Agroinfiltration in Lettuce
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DOI：
10.1007/978-1-4939-3289-4_4
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发表时间：
2016-01-01
期刊：
影响因子：
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通讯作者：
Lai, Huafang
中科院分区：
文献类型：
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作者：
Chen, Qiang;Dent, Matthew;Lai, Huafang
目前的重组蛋白生产系统包括细菌、昆虫和哺乳动物细胞培养物。然而，这些平台的商业规模构建和运营成本昂贵，并且/或生产复杂蛋白质的能力有限。近年来，基于植物的表达系统已成为生产重组蛋白的最佳候选者，因为它们具有高度可扩展性、稳健、安全，并且由于具有真核内膜系统而可以产生复杂的蛋白质。新开发的“解构”病毒载体通过根癌农杆菌（农杆菌渗透）传递，使得基于植物的蛋白质生产具有广泛的应用。绿叶生菜（Lactuca sativa）植物具有深厚的农业基础，是生产药用蛋白质的绝佳宿主。在这里，我们描述了一种生菜农杆菌渗透方法，该方法可以在几天内快速产生高水平的重组蛋白，并且有可能扩大到农业水平。
Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.