Stimulation of murine tumour cell motility by laminin.
Stimulation of murine tumour cell motility by laminin.
复制标题
层粘连蛋白刺激小鼠肿瘤细胞运动。
作者：
R. Situ;E. C. Lee;J. McCoy;J. Varani
从不同的小鼠肿瘤群体中分离出了几个缺乏表面层粘连蛋白的变异细胞系。免疫荧光、免疫过氧化物酶/电子显微镜和免疫沉淀表明，分离出这些变异系的亲本群体含有高水平表达表面层粘连蛋白的细胞。用Boyden小室实验和琼脂糖凝胶实验比较了层粘连蛋白缺失品系与它们各自的亲本群体的活力。在这两种检测中，层粘连蛋白阳性的群体比层粘连蛋白缺乏的品系更具活力。在层粘连蛋白缺失的品系中添加外源层粘连蛋白显著提高了它们的活力。这些观察很有意义，因为细胞的运动被认为有助于肿瘤细胞的转移，而层粘连蛋白阳性的细胞群体是高度致癌和转移的，而层粘连蛋白缺乏的细胞是低成瘤性的，几乎是非转移的。
Several variant cell lines that are deficient in surface laminin have been isolated from heterogeneous murine tumour populations. The parent populations from which these variant lines were isolated contain cells that express high levels of surface laminin as indicated by immunofluorescence, immunoperoxidase/electron microscopy and immunoprecipitation. The laminin-deficient lines were compared with their respective parent populations for motility by both the Boyden Chamber assay and the agarose assay. In both assays, the laminin-positive populations were much more motile than the laminin-deficient lines. The addition of exogenous laminin to the laminin-deficient lines significantly increased their motility. These observations are of interest since cell motility is thought to contribute to tumour cell metastasis, and the laminin-positive cell populations are highly tumorigenic and metastatic, while the laminin-deficient cells are of low tumorigenicity and are virtually non-metastatic.