Culture of human glomerular cells.
Culture of human glomerular cells.
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人肾小球细胞的培养。
DOI：
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发表时间：
1979
影响因子：
6
通讯作者：
M. D. Mills
中科院分区：
文献类型：
--
作者：
T. Oberley;P. Burkholder;M. D. Mills
将人肾小球常规培养在补充有胰岛素和条件培养基的Waymouth培养基中。在成人和婴儿肾脏的培养物中观察到三种细胞类型，但肾小球细胞生长的形态取决于获得肾脏的患者的年龄。来自老年个体的肾小球培养物导致更多的“分化”细胞，但随着培养时间的增加，成人和婴儿肾小球细胞都迅速变得“去分化”。培养肾小球的生长物不含成纤维细胞或肾小管细胞。最后，证明了这些培养的肾小球细胞合成基底膜材料。
Human glomeruli were routinely cultured in Waymouth's medium supplemented with insulin and conditioned medium. Three cell types were seen in the culture of both adult and infant kidneys, but the morphology of the glomerular cellular outgrowths depended on the age of the patient from which the kidney was obtained. Cultures of glomeruli from older individuals resulted in more "differentiated" cells, but both adult and infant glomerular cells rapidly became "dedifferentiated" as the length of time in culture increased. Outgrowths of cultured glomeruli did not contain fibroblasts or tubular cells. Finally, synthesis of basement membrane material by these cultured glomerular cells was demonstrated.