The 724 family of DNA sequences is interspersed about the pericentromeric regions of human acrocentric chromosomes.
The 724 family of DNA sequences is interspersed about the pericentromeric regions of human acrocentric chromosomes.
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DNA 序列 724 家族散布在人类近端着丝粒染色体的着丝粒周围区域。
DOI：
10.1159/000132305
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Patterson,D
中科院分区：
文献类型：
--
作者：
Kurnit,DM;Roy,S;Stewart,GD;Schwedock,J;Neve,RL;Bruns,GA;VanKeuren,ML;Patterson,D
我们描述了组织的复杂，散布724家庭的DNA序列，分布在多个副本的人近端着丝粒染色体的pericentromeric区域。724个家族成员使用有效的基于重组的核苷酸序列同源性测定分离，以筛选人类基因组文库。分离出8个相关但不同的724个家族成员，它们与跨越100，000个碱基对的总共20个不同的人类基因组EcoRlDNA片段杂交。与也位于人类近端着丝粒染色体短臂上的串联成簇的卫星和核糖体DNA序列相比，724个家族成员散布在其中。没有证据表明724家族成员与核糖体或卫星DNA序列之间存在局部散布或同源性。复杂的724家族与核仁组织者区的并置是灵长类进化中最近发生的事件。在人类五条近端着丝粒染色体中的每一条上都有724个家族成员的独特组织，表明724家族在人类核型中继续进化。
We describe the organization of the complex, interspersed 724 family of DNA sequences that is distributed in multiple copies about the pericentromeric region of human acrocentric chromosomes. 724 family members were isolated using an efficient recombination-based assay for nucleotide sequence homology to screen a human genomic library. Eight related but distinct 724 family members were isolated that hybridized to a total of 20 different human-genomicEcoRlDNA fragments spanning 100,000 base pairs. In contrast with tandemly clustered satellite and ribosomal DNA sequences also located on the short arms of human acrocentric chromosomes, 724 family members are interspersed. No evidence for local interspersion or homology between 724 family members and ribosomal or satellite DNA sequences was found. Juxtaposition of the complex 724 family to the nucleolus organizer region was a recent event in primate evolution. The unique organization of 724 family members on each of the five human acrocentric chromosomes indicates that the 724 family continues to evolve within the human karyotype.