Jasmonic acid induced protein response to biophoton emissions and flooding stress in soybean
Jasmonic acid induced protein response to biophoton emissions and flooding stress in soybean
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DOI：
10.1016/j.jprot.2015.12.004
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发表时间：
2016-02-05
影响因子：
3.3
通讯作者：
Komatsu, Setsuko
中科院分区：
文献类型：
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作者：
Kamal, Abu Hena Mostafa;Komatsu, Setsuko
外源植物激素如茉莉酸（JA）和水杨酸（SA）的应用增加了生物光子发射。为了揭示JA和SA处理大豆淹水胁迫响应的分子机制，结合蛋白质组学分析对植物的生物光子排放进行了定量。此外，外源JA处理抑制侧根的生长，并显着降低根重。在JA和JA/SA处理的植物中鉴定的649种蛋白质中，44种是JA处理的植物所特有的，403种是JA/SA处理的植物所特有的，202种是两组之间共有的。这些蛋白质参与应激、信号传导、降解、糖酵解、发酵和激素代谢。谷胱甘肽-S-转移酶，丙氨酸氨基转移酶，苹果酸脱氢酶的丰度下降，但这些酶的活性增加。与此相反，单脱氢抗坏血酸还原酶的丰度和活性增加与JA和SA处理的植物根在淹水胁迫下。这表明，侧根的数量，总根质量，氧化和还原反应和活性氧清除过程中产生的自由基在很大程度上有助于生物光子发射。此外，单脱氢抗坏血酸还原酶，这是参与解毒和控制过氧化氢的水平，可以保护植物细胞免受氧化损伤在flooding.Biological意义：为了了解生物光子发射的来源和分子机制的茉莉酸和水杨酸在大豆植株在淹水条件下，在根中进行的无标记定量技术。根长和重量显着降低茉莉酸和水杨酸的影响，而它抑制生长的侧根在正常条件下使用茉莉酸。最后，通过MAPMAN软件应用程序对鉴定的蛋白质进行功能注释;将其分配到不同的功能类别，例如应激、信号传导、蛋白质、糖酵解、代谢、细胞壁和细胞组织。因此，本研究有助于了解植物体内的光子发射，并了解大豆在淹水胁迫下的分子机制。(C)2015 Elsevier B. V.版权所有。
Biophoton emissions were elevated by the exogenous plant hormone application such as jasmonic (JA) and salicylic acid (SA). To reveal the molecular mechanisms underlying flooding stress responses in soybean treated with JA and SA, biophoton emissions from plants were quantified in combination with proteomic analyses. Furthermore, treatment with exogenous JA inhibited lateral root growth and markedly reduced root weight. Out of 649 proteins identified in the JA- and JA/SA-treated plants, 44 were unique to JA-treated plants, 403 were unique to JA/SA-treated plants, and 202 were shared between the groups. These proteins were involved in stress, signaling, degradation, glycolysis, fermentation, and hormone metabolism. The abundances of glutathione-S-transferase, alanine aminotransferase, and malate dehydrogenase were decreased; however, the activities of these enzymes were increased. In contrast, the abundance and activity of monodehydroascorbate reductase increased in the roots of plants treated with JA and SA under flooding stress. This suggests that the quantity of lateral roots, total root mass, and free radicals generated during oxidation and reduction reactions and reactive oxygen species scavenging largely contribute to biophoton emission. Furthermore, monodehydroascorbate reductase, which is involved in detoxification and controlling hydrogen peroxide levels, may protect plant cells against oxidative damage during flooding.Biological significance: To understand the source of biophoton emission and molecular mechanism by the application of jasmonic and salicylic acid under flooding conditions in soybean plants, the label-free quantitative techniques were performed in roots. Root lengths and weights were significantly reduced by the effect of jasmonic and salicylic acid while it inhibited growth of the lateral roots in normal conditions using the jasmonic acid. Finally, identified proteins were functionally annotated by MAPMAN software application; that were assigned to different functional categories, such as stress, signaling, protein, glycolysis, metabolism, cell wall, and cell organization. Consequently, this study offers to learn the photon emission in plants and to know the molecular mechanism under flooding stress in soybean. (C) 2015 Elsevier B.V. All rights reserved.