GeGen QinLian decoction alleviate influenza virus infectious pneumonia through intestinal flora
GeGen QinLian decoction alleviate influenza virus infectious pneumonia through intestinal flora
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葛根芩连汤通过肠道菌群缓解流感病毒感染性肺炎
DOI：
10.1016/j.biopha.2021.111896
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发表时间：
2021-07-08
影响因子：
7.5
通讯作者：
Chen, Xiaoyin
中科院分区：
文献类型：
--
作者：
Deng, Li;Shi, Yucong;Chen, Xiaoyin
在人类中，流感通常伴随着胃肠炎样症状。葛根芹连汤是一种中药方剂，用于治疗感染性腹泻已有数百年的历史，具有恢复肠道菌群的作用。研究还报告了GQD用于治疗流感患者。然而，调节肠道菌群是否为GQD治疗流感的方法之一尚未得到证实。在本研究中，我们进行了系统的药理学研究，结果表明GQD可能通过多个靶点和途径发挥作用。在流感感染的小鼠中，GQD治疗降低了死亡率和肺部炎症。最重要的是，接受了GQD(FMT-GQD)治疗的小鼠粪便微生物区系移植(FMT)的流感感染小鼠的死亡率和肺部炎症也减少了。GQD治疗或FMT-GQD治疗可恢复肠道菌群，使粘液阿克曼氏菌、硫化弧菌C21_C20和唾液乳杆菌增加，而大肠杆菌减少。FMT-GQD治疗可抑制肠道NOD/RIP2/NF-kappaB信号通路，并影响肠系膜淋巴结和血清中下游相关炎性细胞因子的表达。此外，FMT-GQD治疗通过抑制CD4(+)T细胞的炎性分化而显示出全身保护作用。综上所述，我们的研究表明，GQD可以影响全身免疫，至少部分是通过肠道菌群，从而保护小鼠免受流感病毒感染性肺炎的影响。
Influenza in humans is often accompanied by gastroenteritis-like symptoms. GeGen QinLian decoction (GQD), a Chinese herb formula, has been widely used to treat infectious diarrhea for centuries and has the effect of restoring intestinal flora. Studies have also reported that GQD were used to treat patients with influenza. However, whether regulating the intestinal flora is one of the ways GQD treats influenza has not been confirmed. In present research, we conducted a systemic pharmacological study, and the results showed that GQD may acts through multiple targets and pathways. In influenza-infected mice, GQD treatment reduced mortality and lung inflammation. Most importantly, the mortality and lung inflammation were also reduced in influenza-infected mice that have undergone fecal microbiota transplantation (FMT) from GQD (FMT-GQD) treated mice. GQD treatment or FMT-GQD treatment restores the intestinal flora, resulting in an increase in Akkermansia_muciniphella, Desulfovibrio_C21_c20 and Lactobacillus_salivarius, and a decrease in Escherichia_coli. FMT-GQD treatment inhibited the NOD/RIP2/NF-kappa B signaling pathway in the intestine and affected the expression of downstream related inflammatory cytokines in mesenteric lymph nodes (mLNs) and serum. In addition, FMT-GQD treatment showed systemic protection by restraining the inflammatory differentiation of CD4(+) T cells. In conclusion, our study shows that GQD can affect systemic immunity, at least in part, through the intestinal flora, thereby protect the mice against influenza virus infectious pneumonia.