Expression of plant flavor genes in Lactococcus lactis
Expression of plant flavor genes in Lactococcus lactis
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DOI：
10.1128/aem.01870-06
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发表时间：
2007-03-01
影响因子：
4.4
通讯作者：
van Hylckama Vlieg, Johan E. T.
中科院分区：
文献类型：
--
作者：
Hernandez, Igor;Molenaar, Douwe;van Hylckama Vlieg, Johan E. T.
乳酸菌等乳酸菌因其食品级地位、高效表达和代谢工程工具而成为生产植物生物活性化合物的诱人宿主。利用乳酸链球菌素诱导表达系统，克隆了草莓(Fragaria X Ananassa)的两个基因，分别编码乙醇酰基转移酶(SAAT)和芳樟醇/橙花醇合成酶(FANES)。通过增加乳酸乳杆菌中罕见的tRNA分子tRNA(1)(Arg)的浓度，SAAT的比活力可提高三倍(高达564pmol乙酸辛酯h(-1)mg蛋白质(-1))。在GM17培养基和表达SAAT或FANES的重组乳酸乳杆菌的牛奶发酵试验中，乙酸辛酯(1.9mM)和芳樟醇(85 NM)的产量超过了它们在水中的气味阈值。这些结果说明了乳酸乳杆菌作为食品级表达平台用于从植物中重组生产蛋白质和生物活性化合物的潜力。
Lactic acid bacteria, such as Lactococcus lactis, are attractive hosts for the production of plant-bioactive compounds because of their food grade status, efficient expression, and metabolic engineering tools. Two genes from strawberry (Fragaria x ananassa), encoding an alcohol acyltransferase (SAAT) and a linalool/nerolidol synthase (FaNES), were cloned in L. lactis and actively expressed using the nisin-induced expression system. The specific activity of SAAT could be improved threefold (up to 564 pmol octyl acetate h(-1) mg protein(-1)) by increasing the concentration of tRNA(1)(Arg), which is a rare tRNA molecule in L. lactis. Fermentation tests with GM17 medium and milk with recombinant L. lactis strains expressing SAAT or FaNES resulted in the production of octyl acetate (1.9 mu M) and linalool (85 nM) to levels above their odor thresholds in water. The results illustrate the potential of the application of L. lactis as a food grade expression platform for the recombinant production of proteins and bioactive compounds from plants.