A simple method for measuring interstitial fluid pressure in cancer tissues
A simple method for measuring interstitial fluid pressure in cancer tissues
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DOI：
10.1016/j.mvr.2005.07.003
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发表时间：
2005-07-01
影响因子：
3.1
通讯作者：
Hargens, AR
中科院分区：
文献类型：
--
作者：
Ozerdem, U;Hargens, AR
描述了一种使用聚氨酯传感器尖端导管（Millar）的新方法，该方法可以可靠地测量癌组织中的间质液压力（IFP）。在每次使用之前和之后，传感器在37摄氏度下通过水柱进行校准。校准后，传感器穿过手术针的内腔。在穿透肿瘤期间，传感器保持在针腔中。然后，当针套筒从肿瘤表面撤回时，传感器尖端被引入肿瘤的中心核心。我们的新技术很简单，提供的IFP等于那些由成熟的，芯m针技术提供的IFP。使用我们的新技术，我们比较了NG 2基因敲除小鼠和野生型小鼠皮肤黑色素瘤移植物中的IFP。敲除血管生成和血管生成周细胞上的NG 2蛋白聚糖使黑色素瘤中的间质流体压力从+4.9切割H2O降低至-0.4 cm。水（P = 0.0054 Mann-Whitney U检验）。(c)2005年爱思唯尔公司All rights reserved.
A novel procedure using a polyurethane transducer-tipped catheter (Millar) is described that allows reliable measurement of interstitial fluid pressure (IFP) in cancer tissues. Before and after each use, the transducer is calibrated at 37 degrees C by a water column. After calibration, the transducer is passed through the lumen of a surgical needle. The sensor is kept in the lumen of the needle during penetration into the tumor. The sensor tip is then introduced into the center core of the tumor as the needle sleeve is withdrawn from the tumor surface. Our new technique is simple and provides IFPs equal to those provided by the well-established, wick-m-needle technique. Using our new technique, we compared IFP in skin melanoma grafts in NG2 knockout and wild-type mice. Knocking out NG2 proteoglycan on vasculogenic and angiogenic pericytes reduced interstitial fluid pressure in melanoma from +4.9 cut H2O to -0.4 cm. H2O (P = 0.0054 Mann-Whitney U test). (c) 2005 Elsevier Inc. All rights reserved.