Efficiency of blocking of non-specific interaction of different proteins by BSA adsorbed on hydrophobic and hydrophilic surfaces
Efficiency of blocking of non-specific interaction of different proteins by BSA adsorbed on hydrophobic and hydrophilic surfaces
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DOI：
10.1016/j.jcis.2009.09.007
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发表时间：
2010-01-01
影响因子：
9.9
通讯作者：
Rai, B.
中科院分区：
文献类型：
--
作者：
Jeyachandran, Y. L.;Mielczarski, J. A.;Rai, B.
利用红外反射光谱和光谱模拟，定性和定量地评价了预吸收牛血清白蛋白（BSA）层阻断不同蛋白质在疏水和亲水表面非特异性吸附的效率。当BSA层的表面覆盖率为密实单层的35%时，在疏水性表面上的阻断效率为90-100%，在亲水性表面上的阻断效率为68-100%，对于豆豆蛋白A （Con A）、免疫球蛋白G （IgG）和葡萄球菌蛋白A （SpA）的非特异性吸附。用浓度为1mg /mL的溶液和30min的孵育时间制备BSA层。在通常用于阻断的条件下（孵育时间为12小时，溶液浓度为10 mg/mL）吸附的牛血清白蛋白层表现出竞争性吸附-解吸的阻断活性。这种活性源于BSA-磷酸盐表面复合物的形成，这与吸附的BSA分子的构象相关，有利于阻断。优化BSA层对不同表面和蛋白质的重要性在本研究中得到了解决。(C) 2009爱思唯尔公司版权所有。
The efficiency of a pre-absorbed bovine serum albumin (BSA) layer in blocking the non-specific adsorption of different proteins on hydrophobic and hydrophilic Surfaces was evaluated qualitatively and quantitatively using infrared reflection spectroscopy supported by spectral simulations. A BSA layer with a surface coverage of 35% of a close-packed monolayer exhibited a blocking efficiency of 90-100% on a hydrophobic and 68-100% on a hydrophilic Surface, with respect to the non-specific adsorption of concanavalin A (Con A), immunoglobulin G (IgG), and staphylococcal protein A (SpA). This BSA layer was produced Using a solution concentration of 1 mg/mL and 30 min incubation time. BSA layers that were adsorbed at conditions commonly employed for blocking (a 12 h incubation time and a Solution concentration of 10 mg/mL) exhibited a blocking activity that involved competitive adsorption-desorption. This activity resulted from the formation of BSA-phosphate Surface complexes, which correlated with the conformation of adsorbed BSA molecules that was favourable for blocking. The importance of optimisation 017 the adsorbed BSA layer for different Surfaces and proteins to achieve efficient blocking was addressed in this study. (C) 2009 Elsevier Inc. All rights reserved.