Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum β-lactamases
Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum β-lactamases
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DOI：
10.1099/jmm.0.46909-0
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发表时间：
2007-01-01
影响因子：
3
通讯作者：
Curran, Martin D.
中科院分区：
文献类型：
--
作者：
Birkett, Christopher I.;Ludlam, Hugo A.;Curran, Martin D.
肠杆菌科产 CTX-M 成员的流行率在全球范围内不断增加。描述了一种新颖、多重、实时 TaqMan PCR 测定法来检测和分型 b/aCTX-M 基因，该测定法是对先前描述的技术的改进，减少了测定时间，消除了长时间的 PCR 后处理的需要，并且单个反应容器的便利性。根据 β-内酰胺抗菌谱和 MIC 数据，从临床血液和尿液培养样本中选择了 1279 株肠杆菌科菌株中的 478 株，并使用表型方法测试了超广谱 β-内酰胺酶 (ESBL) 的产生。新的 TaqMan 检测方法在 28 个产 ESBL 分离株中的 21 个中检测到了 b/aCTX-M 基因并对其进行了分型。
The prevalence of CTX-M-producing members of the Enterobacteriaceae is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type b/aCTX-M genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on beta-lactam antibiogram and MIC data, 478 of 1279 Enterobacteriaceae isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum beta-lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed b/aCTX-M genes in 21 of 28 ESBL-producing isolates.