Anti-inflammatory effects of IL-4 and IL-10 on human polymorphonuclear leukocytes
Anti-inflammatory effects of IL-4 and IL-10 on human polymorphonuclear leukocytes
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DOI：
10.3346/jkms.2002.17.1.7
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发表时间：
2002-02-01
影响因子：
4.5
通讯作者：
Moon, CK
中科院分区：
文献类型：
--
作者：
Lee, SW;Hong, YS;Moon, CK
炎症反应受到促炎和抗炎介质协调的严格调节。白细胞介素 4 (IL-4) 和白细胞介素 10 (IL-10) 通常对单核细胞具有生物抗炎作用，但对多形核白细胞 (PMN) 的作用不确定。 PMN 是急性炎症期间宿主防御的第一道细胞反应。为了用生物抗炎介质改变高炎症反应，我们确定了 IL-4 和 IL-10 对人 PMN 的生物抗炎活性。人 PMN 用 IL-4 或 IL-10 预处理，然后用甲酰甲硫氨酰亮氨酰苯丙氨酸 (fMLP) 刺激指定时间。测定每个无细胞上清液中的 H2O2、白细胞介素-8 (IL-8) 和肿瘤坏死因子-α (TNF-α) 的水平。 fMLP 起着典型的促炎剂的作用，并且至少在确定的条件下，小丑调节 TNF 的释放。 IL-4 作为抗炎介质，但 IL-10 对 fMLP 刺激的人 PMN 没有表现出抗炎活性。 IL-4和IL-10具有不同的抗炎机制。也许，IL-10 需要辅助因子才能充当抗炎介质。
Inflammatory responses are strictly regulated by coordination of pro-inflammatory and anti-inflammatory mediators. Interleukin-4 (IL-4) and interleukin-10 (IL-10) have typically the biologic anti-inflammatory effects on monocytes, but uncertain effects on polymorphonuclear leukocytes (PMNs). The PMNs are the first line of cellular response for host defense during acute inflammation. To modify hyper-inflammatory reaction with biologic anti-inflammatory mediators' we have determined the biologic anti-inflammatory activities of IL-4 and IL-10 on human PMNs. Human PMNs were pretreated with IL-4 or IL-10 and then stimulated with formyl methionyl leucyl phenylalanine (fMLP) for times indicated. The level of H2O2, interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-alpha) were determined in the each cell free supernatants. fMLP plays the role of a typical pro-inflammatory agent and, at least in determined conditions, clown-regulated TNF release. IL-4 acts as an anti-inflammatory mediator but IL-10 did not show its anti-inflammatory activities on fMLP-stimulated human PMNs. IL-4 and IL-10 have different anti-inflammatory mechanisms. Perhaps, IL-10 needs co-factors to act as an anti-inflammatory mediator.