CHIMERIC TRANSCRIPTS WITH AN OPEN READING FRAME ARE GENERATED AS A RESULT OF TRANSLOCATION TO THE PVT-1 REGION IN MOUSE B-CELL TUMORS
CHIMERIC TRANSCRIPTS WITH AN OPEN READING FRAME ARE GENERATED AS A RESULT OF TRANSLOCATION TO THE PVT-1 REGION IN MOUSE B-CELL TUMORS
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DOI：
10.1002/ijc.2910590623
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发表时间：
1994-12-15
影响因子：
6.4
通讯作者：
SIWARSKI, D
中科院分区：
文献类型：
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作者：
HUPPI, K;SIWARSKI, D
一些小鼠浆细胞瘤表现出A t（6; 15）染色体易位，其中断点位于小鼠染色体上C-MYC下游的PVT-L基因座内的PVT-L基因座内，在这份报告中，我们显示Pvt-L基因座不表现出等位基因排除，因为Pvt-L转录本继续从t（6; 15）浆细胞瘤中的非翻译等位基因表达。从易位的等位基因中，我们发现嵌入了含有短的57 bp段的PVT-L（称为PVT-LA）的短片段，直接剪接到免疫球蛋白常数区域序列（IG-CK）。这些简短的转录本已通过RNA剪接替换了JK段，并包含来自PVT-LA通过TK的连续开放阅读框（ORF）。由于该PVT-LA/CK转录本均在检查的所有3 t（6; 15）中，无论染色体断点的位置如何小鼠浆细胞瘤。 （c）1994 Wiley-Liss，Inc。*
Some mouse plasmacytomas exhibit a t(6; 15) chromosomal translocation in which the breakpoint resides within the Pvt-l locus located 260 kilobases (kb) downstream of c-myc on mouse chromosome 15. In this report, we show that the Pvt-l locus does not exhibit allelic exclusion in that Pvt-l transcripts continue to be expressed from the non-translocated allele in t(6;15) plasmacytomas. From the translocated allele, we find chimeric transcripts containing a short 57-bp segment of Pvt-l (termed Pvt-la) spliced directly to the immunoglobulin constant region sequence (Ig-Ck). These short transcripts have replaced a Jk segment with a trytophan residue via RNA splicing and contain a continuous open reading frame (ORF) from Pvt-la through tk. Since this Pvt-la/Ck transcript is found in all 3 t(6;15) plasmacytomas examined, regardless of the location of the chromosomal breakpoint, we suggest that the Pvt-la/Ck chimera may have a functional role in the development of mouse plasmacytomas. (C) 1994 Wiley-Liss, Inc.*