Electrokinetic concentration enrichment within a microfluidic device using a hydrogel microplug
Electrokinetic concentration enrichment within a microfluidic device using a hydrogel microplug
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DOI：
10.1039/b509063f
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发表时间：
2005-01-01
期刊：
影响因子：
6.1
通讯作者：
Crooks, RM
中科院分区：
文献类型：
--
作者：
Dhopeshwarkar, R;Li, SA;Crooks, RM
描述了一种用于在微流体装置中浓缩带电分子的简单而有效的方法。该系统的功能组件是在微流体装置的主通道内光聚合的水凝胶微塞。当在水凝胶上施加适当的偏置电压时，带电的分析物分子从源阱向水凝胶移动。然而，与其在微流体通道中的速度相比，分析物通过水凝胶的运输是缓慢的，因此它在水凝胶/溶液界面处浓缩。对于不带电的水凝胶，100 V的偏压导致在150 s内DNA浓度的类似500倍富集，而相同的条件导致荧光素仅富集50倍。当使用带负电荷的水凝胶时，观察到稍低的富集因子。一个定性的模型，提出了考虑到所观察到的行为。
A simple and efficient approach for concentration of charged molecules in microfluidic devices is described. The functional component of the system is a hydrogel microplug photopolymerized within the main channel of a microfluidic device. When an appropriately biased voltage is applied across the hydrogel, charged analyte molecules move from the source well toward the hydrogel. Transport of the analyte through the hydrogel is slow compared to its velocity in the microfluidic channel, however, and therefore it concentrates at the hydrogel/solution interface. For an uncharged hydrogel, a bias of 100 V leads to a similar to 500-fold enrichment of the DNA concentration within 150 s, while the same conditions result in an enrichment of only 50-fold for fluorescein. Somewhat lower enrichment factors are observed when a negatively charged hydrogel is used. A qualitative model is proposed to account for the observed behavior.