New views of cells in 3D: an introduction to electron tomography
New views of cells in 3D: an introduction to electron tomography
复制标题
DOI：
10.1016/j.tcb.2004.11.009
复制
发表时间：
2005-01-01
影响因子：
19
通讯作者：
Mastronarde, D
中科院分区：
文献类型：
--
作者：
McIntosh, R;Nicastro, D;Mastronarde, D
结构细胞生物学最重要的目标是阐明生命过程的机制。膜系统或纤维阵列的结构，这种结构随时间的变化或酶相对于细胞器边界的定位通常可以阐明相关的细胞功能。为了发挥最大的价值，结构研究应该以尽可能高的分辨率提供关于保存完好的样品的各向同性3D信息。电子断层摄影术可以提供许多种细胞和细胞器的信息。
The most important goal of structural cell biology is to elucidate the mechanisms of the processes of life. The structure of a membrane system or fibrous array, the changes in such structures over time or the localizations of enzymes relative to organelle boundaries can often illuminate associated cellular functions. To be of maximal value, structural studies should provide isotropic, 3D information about well-preserved samples at the highest possible resolution. Electron tomography can provide such information about many kinds of cells and organelles.