Changes in bone structure and mass with advancing age in the male C57BL/6J mouse
Changes in bone structure and mass with advancing age in the male C57BL/6J mouse
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DOI：
10.1359/jbmr.2002.17.6.1044
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发表时间：
2002-06-01
影响因子：
6.2
通讯作者：
Majumdar, S
中科院分区：
文献类型：
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作者：
Halloran, BP;Ferguson, VL;Majumdar, S
为了确定小鼠是否随着年龄的增长而失去骨骼以及这些变化是否与人类衰老中观察到的变化相似，我们使用显微计算机断层扫描（muCT）检查了雄性C57 BL/6J小鼠在其寿命期间胫骨干骺端和骨干的变化。松质骨体积分数（BV/TV）在6周龄至24月龄之间下降了60%。损失的特点是减少小梁数量（Tb. N），增加小梁间距（Tb. Sp），并降低连接。各向异性随年龄的增长而减小，结构模型指数随年龄的增长而增大。皮质骨厚度在6周龄至6月龄之间增加，然后持续下降至24月龄（-12%）。皮质骨面积（Ct. Ar）在6至24个月之间保持恒定。无脂体重在12个月时达到峰值，并逐渐下降至24个月。12至24个月的总质量损失达到10%。总体而言，小鼠骨骼质量和结构的年龄相关变化与人类衰老中观察到的变化非常相似。此外，松质骨的快速早期丢失表明，骨丢失不仅与小鼠的衰老有关，而且是作为早期生长的连续体发生的。我们的结论是，C57 BL/6J雄性小鼠可能是一个有用的模型，研究至少在某些方面的年龄相关的骨丢失在人类。
To determine whether the mouse loses bone with aging and whether the changes mimic those observed in human aging, we examined the changes in the tibial metaphysis and diaphysis in the male C57BL/6J mouse over its life span using microcomputed tomography (muCT). Cancellous bone volume fraction (BV/TV) decreased 60% between 6 weeks and 24 months of age. Loss was characterized by decreased trabecular number (Tb.N), increased trabecular spacing (Tb.Sp), and decreased connectivity. Anisotropy decreased while the structure model index increased with age. Cortical bone thickness increased between 6 weeks and 6 months of age and then decreased continuously to 24 months (- 12 %). Cortical bone area (Ct.Ar) remained constant between 6 and 24 months. Fat-free weight reached a peak at 12 months and gradually declined to 24 months. Total mass lost between 12 and 24 months reached 10%. Overall, the age-related changes in skeletal mass and architecture in the mouse were remarkably similar to those seen in human aging. Furthermore, the rapid early loss of cancellous bone suggests that bone loss is not just associated with old age in the mouse but rather occurs as a continuum from early growth. We conclude that the C57BL/6J male mouse maybe a useful model to study at least some aspects of age-related bone loss in humans.