An investigation of the contribution of the extraosseous tissues to the diaphyseal fracture callus using a rabbit tibial fracture model and in situ immunocytochemical localisation of osteocalcin.
An investigation of the contribution of the extraosseous tissues to the diaphyseal fracture callus using a rabbit tibial fracture model and in situ immunocytochemical localisation of osteocalcin.
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DOI：
10.1097/00005131-199206000-00010
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发表时间：
1992-01-01
影响因子：
2.3
通讯作者：
Gregg, P J
中科院分区：
文献类型：
--
作者：
Stafford, H;Oni, O O;Gregg, P J
利用兔胫骨骨折模型和骨钙素免疫细胞化学方法研究了骨外组织对骨干骨折骨痂的作用。通过扩髓和钉入截骨术并切除截骨术两侧2 cm的骨膜，分离骨外组织进行研究。分别在术后1周和2周从愈合的骨折中获得的标本进行脱钙和骨钙素染色，骨特异性蛋白，使用间接免疫过氧化物酶法。阳性染色的成骨细胞似乎完全来自剩余的骨膜。
The extraosseous tissue contribution to diaphyseal fracture callus has been investigated using a rabbit tibial fracture model and osteocalcin immunocytochemistry. The extraosseous tissues were isolated for study by reaming and nailing an osteotomy and excising 2 cm of periosteum on either side of the osteotomy. Specimens obtained from the healing fractures at 1 and 2 weeks after operation, respectively, were decalcified and stained for osteocalcin, a bone-specific protein, using an indirect immunoperoxidase method. The positively stained osteogenic cells appeared to be derived exclusively from the remnant of the periosteum.