DETECTION OF HSP-72 SYNTHESIS AFTER ACOUSTIC OVERSTIMULATION IN RAT COCHLEA
DETECTION OF HSP-72 SYNTHESIS AFTER ACOUSTIC OVERSTIMULATION IN RAT COCHLEA
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DOI：
10.1016/0378-5955(93)90102-7
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发表时间：
1993-09-01
期刊：
影响因子：
2.8
通讯作者：
ALTSCHULER, RA
中科院分区：
文献类型：
--
作者：
LIM, HH;JENKINS, OH;ALTSCHULER, RA
本研究的目的是确定高强度声刺激是否会诱导大鼠耳蜗HSP 72。将动物暴露于110 dB SPL宽带噪声中1.5 h，并在刺激后4、6和8 h处死。采用免疫细胞化学和蛋白质印迹法检测耳蜗组织中HSP 72的表达.蛋白质印迹显示出一个强烈的72 kD带在噪声暴露的动物相比，一个非常轻的带在非刺激的对照动物。免疫细胞化学结果显示，噪声诱导外毛细胞HSP 72免疫反应阳性染色。内毛细胞仅少量染色，螺旋神经节细胞未染色。这些结果表明，声音过度刺激可诱导大鼠耳蜗外毛细胞HSP 72的表达。HSP 72可作为细胞应激和潜在损伤的标志物，并可参与保护免受损伤。
The purpose of this study was to determine if high intensity acoustic stimulation would induce HSP 72 in rat cochlea. The animals were exposed to 110 dB SPL broad band noise for 1.5 h and sacrificed 4, 6 and 8 h after stimulation. Immunocytochemistry and western blotting were used to detect the expression of HSP 72 in the cochlear tissues. Western blots showed an intense 72 kD band in the noise exposed animals compared to a very light band in non-stimulated control animals. Immunocytochemical results in the cochlea revealed noise induced HSP 72 immunoreactive staining of outer hair cells. Only a few immunoreactive stained inner hair cells were seen and spiral ganglion cells were not stained. These results indicate that acoustic overstimulation can induce the expression of HSP 72 in outer hair cells of the rat cochlea. HSP 72 may serve as a marker for cellular stress and potential damage and may be involved in protection from insult.