Periodontal disease and detection of human herpesviruses in saliva and gingival crevicular fluid of chronic kidney disease patients
Periodontal disease and detection of human herpesviruses in saliva and gingival crevicular fluid of chronic kidney disease patients
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DOI：
10.1002/jper.19-0583
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发表时间：
2020-02-26
影响因子：
4.3
通讯作者：
Braz-Silva, Paulo H.
中科院分区：
文献类型：
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作者：
Pallos, Debora;Ruivo, Gilson F.;Braz-Silva, Paulo H.
背景慢性肾脏病（CKD）患者不能维持正常水平的蛋白质代谢产物、血压和红细胞压积。牙周病涉及炎症性破坏过程。机会性病毒的鉴定非常重要，因为它们与合并症有关。本研究的目的是分析存在的人类疱疹病毒唾液和龈沟液（GCF）从CKD患者。方法131个人被分为根据CKD的阶段：第1组（肌酐清除率> 75 mL/min）无肾脏疾病的患者（n = 24）;第2组（肌酐清除率11-75 mL/min）肾病患者（n = 67）;第3组（肌酐清除率< 10 mL/min）血液透析患者（n = 40）。评估牙周病的参数。结果单纯疱疹病毒Ⅰ型（HSV-1）在唾液中的检出率为9%，龈沟液中为5%，EB病毒在唾液中的检出率为36%，龈沟液中为39%，人巨细胞病毒（HCMV）在龈沟液中的检出率为11%，水痘带状疱疹病毒在唾液中的检出率为6%，龈沟液中为3%，人疱疹病毒6型（HHV-6）在唾液中的检出率为6%，在龈沟液中的检出率为2%，HHV-7型在唾液中的检出率为44%，在龈沟液中的检出率为8%。其中46.48%的患者患有严重的牙周炎。HSV-1和HCMV之间的统计学显着关联被发现在血液透析患者和严重的牙周炎也更频繁其中。结论这些研究结果表明，重要的牙周疾病的评价和检测疱疹病毒在CKD患者的炎症过程中观察到这些临床条件可能会恶化牙周疾病和CKD的过程。
Background Patients with chronic kidney disease (CKD) have inability to maintain the normal levels of protein metabolism products, blood pressure and hematocrit. Periodontal disease involves an inflammatory destructive process. Identification of opportunistic viruses is extremely important as they are associated with co-morbidities. The objective of this study was to analyse the presence of human herpesviruses in saliva and gingival crevicular fluid (GCF) from patients with CKD.Methods One hundred and thirty one individuals were divided depending on the stage of CKD: Group 1 (clearance of creatinine > 75 mL/min) patients with no renal disease (n = 24); Group 2 (clearance of creatinine of 11-75 mL/min) patients with renal disease (n = 67); Group 3 (clearance of creatinine < 10 mL/min) patients on hemodialysis (n = 40). The parameters of periodontal disease were evaluated. The viral detection was assessed by PCR.Results considering the three groups, the prevalence of herpes simplex virus 1 (HSV-1) were 9% in saliva and 5% in GCF; Epstein-Barr virus 36% in saliva and 39% in GCF; human cytomegalovirus (HCMV) 11% in GCF; varicella zoster virus 6% in saliva and 3% in GCF; of human herpesvirus-6 (HHV-6) 6% in saliva and 2% in GCF; and HHV-7 44% in saliva and 8% in GCF. Of these patients, 46.48% presented with severe periodontitis. A statistically significant association between HSV-1 and HCMV was found in hemodialysis patients and severe periodontitis was also more frequent among them.Conclusion These findings show the importance of evaluating the periodontal disease and detecting herpesviruses in patients with CKD as the inflammatory process observed in these clinical conditions may worsen the course of both periodontal disease and CKD.