Silk nano-discs: A natural material for cancer therapy
Silk nano-discs: A natural material for cancer therapy
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DOI：
10.1002/bip.23231
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发表时间：
2018-11-01
期刊：
影响因子：
2.9
通讯作者：
Katiyar, Vimal
中科院分区：
文献类型：
--
作者：
Patwa, Rahul;Soundararajan, Narendren;Katiyar, Vimal
本文展示了结晶丝纳米盘（CSN），其具有良好控制的形态，其在磁化时产生磁性结晶丝纳米盘，从而在治疗学和植入物中替代金属模板如金、银等的两个突出的替代物。分离的富含β-折叠的双链CSN具有类似于50 nm的直径、高结晶度（> 90%），并且是不溶性的，但在水溶液中提供良好的溶解性和稳定性。官能化CSN与聚（乳酸）的熔融共混暨静电纺丝产生具有体外细胞细胞相容性的生物相容性纳米纤维基支架，其具有改善的细胞粘附和增殖。姜黄素是一种天然存在的抗癌药物，其释放行为的评估显示持续释放超过25天，对人宫颈癌细胞表现出有效的细胞毒性。此外，姜黄素和热疗的组合作用使细胞生长降低了约63%。由于静电纺丝过程中有效的纤维拉伸过程，CSN衍生的磁性纳米颗粒的排列可以改善对BHK-21细胞的细胞相容性，从而提高癌症治疗的功效。
The article demonstrates the crystalline silk nano-discs (CSNs), with well-controlled morphology, which upon magnetization, yields magnetic crystalline silk nano-discs, making both prominent alternatives for replacing metal templates such as gold, silver, and so on in therapeutics and implants. The isolated beta-sheet-rich discotic CSNs have similar to 50 nm diameter, high crystallinity (> 90%), and are insoluble but provide good dispersibility and stability in aqueous solutions. The melt blending-cum-electrospinning of functionalized CSN with poly(lactic acid) results in biocompatible nanofiber-based scaffolds having in vitro cell cytocompatibility with improved cell adhesion and proliferation. The assessment of release behavior of curcumin, a naturally occurring anticancer drug, shows sustained release over 25 days exhibiting effective cytotoxicity against human cervical cancer cells. Further, combined effect of curcumin and hyperthermia reduced the cell growth by similar to 63%. Alignment of CSN-derived magnetic nanoparticles due to effective fiber drawing process during electrospinning could improve cytocompatibility against BHK-21 cells, and therefore efficacy for cancer therapy.