A new procedure for experimental autoimmune uveitis with small uveitogenic peptides.
A new procedure for experimental autoimmune uveitis with small uveitogenic peptides.
复制标题
使用小葡萄膜肽治疗实验性自身免疫性葡萄膜炎的新方法。
DOI：
10.1080/09273940600899684
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发表时间：
2006
影响因子：
3.3
通讯作者：
Yamamoto,Nobuto
中科院分区：
文献类型：
--
作者：
Sery,TheodoreW;Su,Ying-Hsiu;EagleJr,Ralph;Ueda,Masumi;Yamamoto,Nobuto
目的：利用光感受器间视黄醇结合蛋白 (IRPB) 的极小片段肽（12-30 个氨基酸残基）演示实验性自身免疫性葡萄膜炎 (EAU)。方法：将极小的片段肽（编号 854、888、907 和 1057）与热灭活的 A 组链球菌细胞缀合，并单次静脉注射给 Lewis 大鼠。非致葡萄膜肽950也与热灭活的链球菌缀合并施用。施用小肽和链球菌的混合物作为与链球菌缀合的肽的对照。结果：致葡萄膜肽/链球菌缀合物在葡萄膜、视网膜和松果体中产生葡萄膜炎炎症反应。施用小肽和链球菌细胞的混合物以及与链球菌缀合的非致葡萄膜肽950不会产生自身免疫性葡萄膜炎。结论：由于小肽和链球菌细胞的混合物不会产生自身免疫性葡萄膜炎，缀合的链球菌细胞为巨噬细胞吞噬非常小的致葡萄膜IRBP肽提供了载体。随后抗原从巨噬细胞呈递至淋巴细胞形成自身免疫性葡萄膜炎。肽 888 是包含主要葡萄膜发生结构域的四种 IRBP 肽之一，被证明对葡萄膜炎的发展最有效。
Purpose: Demonstration of experimental autoimmune uveitis (EAU) with extremely small, fragmented peptides (12–30 amino acid residues) of interphotoreceptor retinoid-binding protein (IRPB).Method: Very small fragmented peptides (no. 854, 888, 907, and 1057) were conjugated to heat-killed Group AStreptococcuscells and administered as a single intravenous injection to Lewis rats. A non-uveitogenic peptide 950 was also conjugated to heat-killedStreptococcusand administered. Administration of a mixture of small peptides andStreptococcuswas a control for the peptides conjugated withStreptococcus.Results: The uveitogenic peptide/Streptococcusconjugates produced uveitis inflammatory responses in the uvea, retina and pineal gland. Administration of mixtures of small peptides and Streptococcus cells, and a non-uveitogenic peptide 950 conjugated withStreptococcusdid not produce autoimmune uveitis.Conclusions: Since mixtures of small uveitogenic peptides and Streptococcal cells did not develop autoimmune uveitis, conjugated Streptococcal cells provided a vehicle for macrophage phagocytosos of very small uveitogenic IRBP peptides. Subsequent antigen presentation from macrophages to lymphocytes developed autoimmune uveitis. Peptide 888, one of four IRBP peptides that encompass the major uveitogenic domain, proved to be the most effective in development of uveitis.