PHENOTYPIC CHARACTERIZATION AND MOLECULAR MAPPING OF THE ARABIDOPSIS-THALIANA LOCUS RPP5, DETERMINING DISEASE RESISTANCE TO PERONOSPORA-PARASITICA
PHENOTYPIC CHARACTERIZATION AND MOLECULAR MAPPING OF THE ARABIDOPSIS-THALIANA LOCUS RPP5, DETERMINING DISEASE RESISTANCE TO PERONOSPORA-PARASITICA
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DOI：
10.1046/j.1365-313x.1993.04050821.x
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发表时间：
1993-11-01
期刊：
影响因子：
7.2
通讯作者：
JONES, JDG
中科院分区：
文献类型：
--
作者：
PARKER, JE;SZABO, V;JONES, JDG
Peronospora寄生在十字花科植物上引起霜霉病。鉴定出一株寄生拟南芥（P. parasitica）分离物（记为NoCO2）可侵染陆地种哥伦比亚（Col-0）的拟南芥植株，而不侵染陆地种直立Landsberg erecta （La-er）的植株。对La-er与Col-0杂交F2植株的分离分析表明，该抗性为单位点遗传，标记为RPP5。对接种后的La-er和Col-0子叶的宏观和显微检查表明，真菌在La-er的生长受到限制，与真菌试图渗透点直接接触的植物细胞大量胼胝质积累和死亡。La-er x col0 F1植物在真菌发育的各个方面都表现出中等抗性反应，表明RPP5在其作用中处于半优势地位。利用La-er和Col-0之间产生的F8重组自交系，将RPP5定位到一个狭窄的区间(
Peronospora parasitica causes downy mildew on crucifers. An isolate of P. parasitica (denoted NoCO2) was identified that infected Arabidopsis plants of the land race Columbia (Col-0) but not plants of land race Landsberg erecta (La-er). Segregation analysis of F2 plants derived from a La-er x Col-0 cross established that the resistance was inherited as a single locus, denoted RPP5. Macroscopic and microscopic examinations of inoculated La-er and Col-0 cotyledons showed that restriction of fungal growth in La-er was accompanied by massive callose accumulation and death of plant cells in direct contact with points of attempted fungal penetration. La-er x Col-0 F1 plants exhibited an intermediate resistance response in all aspects of fungal development, indicating that RPP5 is semi-dominant in its action. F8 recombinant inbred lines generated between La-er and Col-0 were used to map RPP5 to a narrow interval (