EXPRESSION OF SNAIL2, A 2ND MEMBER OF THE ZEBRAFISH SNAIL FAMILY, IN CEPHALIC MESENDODERM AND PRESUMPTIVE NEURAL CREST OF WILD-TYPE AND SPADETAIL MUTANT EMBRYOS
EXPRESSION OF SNAIL2, A 2ND MEMBER OF THE ZEBRAFISH SNAIL FAMILY, IN CEPHALIC MESENDODERM AND PRESUMPTIVE NEURAL CREST OF WILD-TYPE AND SPADETAIL MUTANT EMBRYOS
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DOI：
10.1006/dbio.1995.0007
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发表时间：
1995-11-01
影响因子：
2.7
通讯作者：
POSTLETHWAIT, JH
中科院分区：
文献类型：
--
作者：
THISSE, C;THISSE, B;POSTLETHWAIT, JH
新发现的基因 snail2（编码 Snail 家族的锌指蛋白）的转录本首先出现在原肠胚斑马鱼胚胎的头侧中内胚层细胞中。原肠胚形成结束时，snail2 RNA 积聚在外胚层细胞的区域中，该区域标志着表皮上皮和神经板之间的边界，并包括神经嵴的前体。在体节发生过程中，snail2 的表达仅限于神经嵴。因此，snail2 是已知最早在斑马鱼胚胎神经嵴中特异性表达的基因之一。由于 snail2 在中内胚层中表达，中内胚层是一种组织层，已知其在树干中的会聚在铲尾突变纯合胚胎中发生改变，因此我们检查了铲尾胚胎中的 snail2 表达。在这些突变体中，表达 snail2 的头侧中内胚层细胞数量大大减少，并且含有 snail2 且不含尾部转录本的细胞在中轴中胚层中的分布比正常情况更广泛。此外，在原肠胚形成结束时，胚胎比正常胚胎短。这说明，除了原肠胚形成过程中躯干旁轴中内胚层不能正常会聚外，铲尾还会影响胚胎的伸长以及躯干和头部的轴向和侧向中内胚层的会聚。 (C) 1995 学术出版社
Transcripts of a newly discovered gene called snail2, encoding a zinc finger protein of the Snail family, first appear in rows of cephalic mesendodermal cells in gastrulating zebrafish embryos. At the end of gastrulation, snail2 RNA accumulates in a domain of ectodermal cells that mark the border between the epidermal epithelium and the neural plate and includes precursors of the neural crest. During somitogenesis, snail2 expression becomes restricted to neural crest. snail2 is thus one of the earliest genes yet known to be specifically expressed in neural crest in zebrafish embryos. Since snail2 is expressed in mesendoderm, a tissue layer whose convergence in the trunk is known to be altered in embryos homozygous for the spadetail mutation, we examined snail2 expression in spadetail embryos. In these mutants, the number of cephalic mesendodermal cells expressing snail2 is strongly reduced and the distribution of cells containing snail2 and no tail transcripts in the axial mesoderm is much broader than normal. Moreover, the embryos are shorter than normal at the end of gastrulation. This shows that, in addition to the failure of paraxial mesoderm to converge normally in the trunk during gastrulation, spadetail also affects the elongation of the embryo and the convergence of axial and lateral mesendoderm in both trunk and head. (C) 1995 Academic Press, Inc.