Identification and immunohistochemical localization of macrophage migration inhibitory factor in human cornea
Identification and immunohistochemical localization of macrophage migration inhibitory factor in human cornea
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DOI：
10.1016/0014-5793(96)00386-9
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发表时间：
1996-05-06
期刊：
影响因子：
3.5
通讯作者：
Nishihira, J
中科院分区：
文献类型：
--
作者：
Matsuda, A;Tagawa, Y;Nishihira, J
本实验检测了巨噬细胞移动抑制因子（macrophagemigrationinhibitoryfactor，MIF）mRNA在人角膜中的表达，并对其进行了免疫组织化学定位。逆转录-聚合酶链反应（RT-PCR）分析表明，MIF mRNA在角膜上皮细胞和内皮细胞均有表达。用重组人MIF免疫家兔制备多克隆抗体，免疫组化显示MIF主要分布于角膜上皮基底细胞和内皮细胞。MIF存在于角膜的这些细胞中的事实表明，MIF可能在角膜细胞免疫和细胞分化中起重要作用。
We identified macrophage migration inhibitory factor (MIF) mRNA expression in human cornea, and demonstrated its immunohistological localization, Reverse transcription-polymerase chain reaction analysis revealed that MIF mRNA was expressed in both the corneal epithelial and endothelial cells. Immunohistochemical study using the polyclonal antibody prepared from immunizing a rabbit with human recombinant MIF showed that MIF was present in the basal cells of corneal epithelium and endothelial cells. The fact that MIF exists in those cells of the cornea indicates that MIF may play an important role in corneal cell immunity and cellular differentiation.