Changes in myosin heavy-chain isoform synthesis of chronically stimulated rat fast-twitch muscle.
Changes in myosin heavy-chain isoform synthesis of chronically stimulated rat fast-twitch muscle.
复制标题
长期刺激大鼠快肌的肌球蛋白重链亚型合成的变化。
DOI：
10.1111/j.1432-1033.1992.tb16669.x
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
D. Pette
中科院分区：
文献类型：
--
作者：
A. Termin;D. Pette
采用慢性低频电刺激方法，研究大鼠快缩肌对神经肌肉活动增加的适应性潜能。使用[35 S]甲硫氨酸体内标记技术，在翻译水平上研究了肌球蛋白重链亚型HCIIb与HCIId和HCIIa的顺序交换。在刺激开始后两天，已经可检测到重链同种型合成的改变，即HCIIb标记的减少伴随HCIId/IIa标记的增强。该时间过程对应于先前观察到的HCIIb和HCIIa mRNA量的改变。然而，HCIIb和HCIId/IIa的相对蛋白量的显着变化，仅在8天的刺激期后记录。蛋白质水平的这种延迟被解释为与HCIIb的缓慢周转有关，HCIIb的缓慢周转是从其在长期刺激肌肉中的衰减估计的，近似值为14.7天。因此，蛋白质降解似乎是粗丝重塑过程中的重要翻译后调节步骤。
Chronic low-frequency stimulation was used for studying the adaptive potential of rat fast-twitch muscle to increased neuromuscular activity. The sequential exchange of myosin heavy chain isoforms HCIIb with HCIId and HCIIa was studied at the translational level using an in-vivo-labeling technique with [35S]methionine. Alterations in heavy chain isoform synthesis, i.e. a decrease in the labeling of HCIIb concomitant with an enhanced labeling of HCIId/IIa, were detectable already two days after the onset of stimulation. This time course corresponds to the previously observed alterations in the amounts of HCIIb and HCIIa mRNAs. However, significant changes in the relative protein amounts of HCIIb and HCIId/IIa were recorded only after an 8-day stimulation period. This delay at the protein level was interpreted to relate to the slow turnover of HCIIb which was estimated from its decay in long-term stimulated muscles with an approximate value of 14.7 days. Therefore, protein degradation seems to be an important post-translational regulatory step in the remodeling process of the thick filament.
DOI：
10.1016/s0021-9258(18)77444-9
发表时间：
1990-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Kirschbaum;H. Kucher;A. Termin;A. Kelly;D. Pette
通讯作者：
B. Kirschbaum;H. Kucher;A. Termin;A. Kelly;D. Pette