Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production
Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production
复制标题
DOI：
10.1016/j.cep.2009.03.006
复制
发表时间：
2009-06-01
影响因子：
4.3
通讯作者：
Del Borghi, Marco
中科院分区：
文献类型：
--
作者：
Converti, Attilio;Casazza, Alessandro A.;Del Borghi, Marco
用于生产生物柴油的生物材料的可能来源由微藻代表，特别是由它们的脂质含量代表。研究了温度和氮浓度对微拟球藻（Nannochloropsis oculata）和普通小球藻（Chlorella vulgaris）油脂含量的影响，为微拟球藻和普通小球藻作为生物柴油生产新原料提供依据。此外，还研究了各种脂质提取方法。提取的脂质进行了定量和定性分析，重量和气相色谱法，分别，以检查其适用性，根据欧洲标准的生物柴油。微藻的脂质含量受到测试参数变化的强烈影响;事实上，温度从20摄氏度增加到25摄氏度，N. oculata（7.90%~ 14.92%），而温度从25 ℃升高到30 ℃，则使其脂肪含量下降。寻常型为14.71 ~ 5.90%。另一方面，培养基中的氮浓度降低了75%。相对于生长的最适值，N. oculata为7.90 ~ 15.31%;寻常型为5.90 ~ 16.41%。(C)2009爱思唯尔有限公司版权所有。
A possible source of biological material for the production of biodiesel is represented by microalgae, in particular by their lipid content. The aim of the present work was to study of the effects of temperature and nitrogen concentration on the lipid content of Nannochloropsis oculata and Chlorella vulgaris in view of their possible utilization as novel raw materials for biodiesel production. In addition, various lipid extraction methods were investigated. The extracted lipids were quantitatively and qualitatively analyzed by gravimetric and gas chromatographic methods, respectively, in order to check their suitability according to the European standards for biodiesel. The lipid content of microalgae was strongly influenced by the variation of tested parameters; indeed, an increase in temperature from 20 to 25 degrees C practically doubled the lipid content of N. oculata (from 7.90 to 14.92%), while an increase from 25 to 30 degrees C brought about a decrease of the lipid content of C. vulgaris from 14.71 to 5.90%. On the other hand, a 75% decrease of the nitrogen concentration in the medium. with respect to the optimal values for growth, increased the lipid fractions of N. oculata from 7.90 to 15.31% and of C. vulgaris from 5.90 to 16.41%, respectively. (C) 2009 Elsevier B.V. All rights reserved.