Exogenous infusion of short-chain fatty acids can improve intestinal functions independently of the gut microbiota
Exogenous infusion of short-chain fatty acids can improve intestinal functions independently of the gut microbiota
复制标题
外源性输注短链脂肪酸可以独立于肠道微生物群改善肠道功能
DOI：
10.1093/jas/skaa371
复制
发表时间：
2020
影响因子：
3.3
通讯作者：
Daiwen Chen
中科院分区：
文献类型：
--
作者：
Hua Zhou;Jing Sun;Liangpeng Ge;Zuohua Liu;Hong Chen;Bing Yu;Daiwen Chen
抽象的。本实验旨在探讨外源性注入短链脂肪酸(SCFA)对无菌猪生长发育和肠道功能的影响。将12头子宫切除新生仔猪饲养在6个无菌隔离器中。所有仔猪均饲喂经Co60-γ辐照的无菌奶粉21d，然后切换到无菌喂养21d。在第二个21d期间，饲喂无菌生理盐水25mL/kg/d，饲喂SCFAs混合物(分别为45、15和11 mm)。观察SCFA组大鼠血清和肠道中SCFAs浓度，回肠G蛋白偶联受体43信使核糖核酸(MRNA)丰度增加(P<0.05)。同时，灌胃SCFAs可增加空肠和血清中胰高血糖素样肽-2的含量(P<；0.05)，并有增加回肠绒毛高度的趋势(P<；0.10)。刺激脂肪酶、胰酶、蔗糖酶、乳糖酶、Na+-K+-三磷酸腺苷酶和钙镁离子-三磷酸腺苷酶活性，上调空肠组织中溶质载体家族7(SLC7A1)和再生蛋白(REG)-ΙΙΙγ的基因表达。此外，SCFAs还能降低空肠、回肠和结肠组织中IL-1mRNA和IL-6mRNA的丰度(P<0.05)，增加血液中白细胞、中性粒细胞和淋巴细胞的数量(P<0.05)。总体而言，外源注入单链脂肪酸可能通过促进肠道发育和吸收功能，增强肠道免疫功能来改善肠道健康，这些影响不依赖于肠道微生物区系而发生。
Abstract. The present experiment was conducted to investigate the effects of exogenously infused short-chain fatty acids (SCFAs) on the growth development and intestinal functions in a germ-free (GF) pig model. Twelve hysterectomy-derived newborn piglets were reared in six sterile isolators. All piglets were hand-fed Co60-γ-irradiated sterile milk powder for 21 d and then were switched to sterile feed for another 21 d. During the second 21-d period, GF piglets (n = 6) were orally infused with 25 mL/kg sterile saline per day, and SCFA piglets (n = 6) were orally infused with 25 mL/kg SCFAs mixture (acetic, propionic, and butyric acids, 45, 15, and 11 mM, respectively) per day. We observed the concentrations of SCFAs in serum and intestine, and the messenger ribonucleic acid (mRNA) abundance of G-protein-coupled receptor-43 in the ileum was increased (P < 0.05) in the SCFA group. Meanwhile, oral infusion of SCFAs enhanced (P < 0.05) the contents of glucagon-like peptide-2 in the jejunum and serum and tended to increase the villi height in the ileum (P < 0.10). Besides, the activities of lipase, trypsin, sucrase, lactase, Na+-K+-adenosine triphosphatase ([ATPase] P < 0.05), and Ca2+-Mg2+-ATPase (P < 0.10) were stimulated and the mRNA expressions of solute carrier family 7 (SLC7A1) and regeneration protein (REG)-ΙΙΙ γ in the jejunum (P < 0.05) were upregulated in the SCFA group. Additionally, SCFAs infusion downregulated the mRNA abundances of interleukin (IL)-1β and IL-6 in the jejunum, ileum, or colon (P < 0.05) and increased the counts of white blood cell, neutrophils, and lymphocyte in the blood (P < 0.05). Collectively, exogenous infusion of SCFAs might improve intestinal health through promoting intestinal development and absorption function, and enhancing intestinal immune function, and these effects were occur independently of the gut microbiota.