A systematic analysis of human CHMP protein interactions: Additional MIT domain-containing proteins bind to multiple components of the human ESCRT III complex
A systematic analysis of human CHMP protein interactions: Additional MIT domain-containing proteins bind to multiple components of the human ESCRT III complex
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DOI：
10.1016/j.ygeno.2006.04.003
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发表时间：
2006-09-01
期刊：
影响因子：
4.4
通讯作者：
Sanderson, Christopher M.
中科院分区：
文献类型：
--
作者：
Tsang, Hilda T. H.;Connell, James W.;Sanderson, Christopher M.
在酿酒酵母中，6种密切相关的蛋白质（Did 2 p、Vps 2 p、Vps 24 p、Vps 32 p、Vps 60 p、Vps 20 p）形成延伸的ESCRT III复合物的一部分。该复合物是多泡体形成和内化跨膜受体蛋白降解所必需的。相比之下，人类基因组编码10种同源蛋白质（CHMP 1A（批准的基因符号PCOLN 3）、1B、2A、2B、3（批准的基因符号VPS 24）、4A、4 B、4C、5和6）。在这项研究中，我们已经进行了一系列的蛋白质相互作用的实验，以产生一个更全面的人类CHMP蛋白质相互作用网络的图片。我们的研究结果描述了人类ESCRT III复合物的已知成分之间的新型相互作用，并确定了一系列推定的结合伙伴，这可能表明人类CHMP蛋白的功能可能受到调节的新途径。特别是，我们表明，两个进一步的MIT结构域的蛋白质（AMSH/STAMBP和AM 129531）相互作用的人ESCRT III复合物的多个组件。(c)2006年爱思唯尔公司All rights reserved.
In Saccharomyces cerevisiae 6 closely related proteins (Did2p, Vps2p, Vps24p, Vps32p, Vps60p, Vps20p) form part of the extended ESCRT III complex. This complex is required for the formation of multivesicular bodies and the degradation of internalized transmembrane receptor proteins. In contrast the human genome encodes 10 homologous proteins (CHMP1A (approved gene symbol PCOLN3), 1B, 2A, 2B, 3 (approved gene symbol VPS24), 4A, 4B, 4C, 5, and 6). In this study we have performed a series of protein interaction experiments to generate a more comprehensive picture of the human CHMP protein-interaction network. Our results describe novel interactions between known components of the human ESCRT III complex and identify a range of putative binding partners, which may indicate new ways in which the function of human CHMP proteins may be regulated. In particular, we show that two further MIT domain-containing proteins (AMSH/STAMBP and LOC 129531) interact with multiple components of the human ESCRT III complex. (c) 2006 Elsevier Inc. All rights reserved.