Metabolites in stored platelets associated with platelet recoveries and survivals
Metabolites in stored platelets associated with platelet recoveries and survivals
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DOI：
10.1111/trf.13631
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发表时间：
2016-08-01
期刊：
影响因子：
2.9
通讯作者：
Fu, Xiaoyun
中科院分区：
文献类型：
--
作者：
Zimring, James C.;Slichter, Sherrill;Fu, Xiaoyun
血小板输注（PLT）是许多临床环境中的常见疗法。然而，很好地理解，在PLT储存的良好程度以及因此输注的产品的质量方面存在实质性的供体与供体的差异。这种变化的基础知之甚少，并且可以评估PLT单位的输血后性能的指标有限。它已被反复证明，无数的生物学变化发生在血小板储存，然而，这些变化与储存的血小板的质量和/或机制参与血小板功能仍然undertaked.Study设计和METHODSThe目前的研究测试储存的血小板从21个正常受试者，结合高分辨率代谢组学储存的血小板与体内血小板回收率和生存。与输血后PLT活力相关的单个分析物和代谢途径均被确定。咖啡因代谢产物与PLT恢复不良相关;咖啡因代谢在PLT袋中未进行，并保持在储存前水平。酰基肉毒碱，特别是脂肪酸代谢产物，和氧化脂肪酸与血小板存活率差。在PLT储存过程中的无数代谢变化中，这些是第一次报道的代谢发现，开始区分哪些变化对于输血后PLT性能具有功能重要性。结论总之，这些发现为PLT储存病变的功能生物学提供了新的机制见解，并确定了改变供体环境的潜在靶点（例如，咖啡因消耗量）以及储存的人类PLT的质量评估指标。
BACKGROUNDTransfusion of platelets (PLTs) is a common therapy in a number of clinical settings. However, it is well understood that there is substantial donor-to-donor variation in how well PLTs store and thus the quality of the products that are transfused. The basis of such variation is poorly understood, and there are limited metrics by which units of PLTs can be assessed for their posttransfusion performance. It has repeatedly been demonstrated that myriad biologic changes take place during PLT storage; however, which of the changes correlate with quality of the stored PLTs and/or are mechanistically involved in PLT function remains undetermined.STUDY DESIGN AND METHODSThe current study tested stored PLTs from 21 normal subjects, combining high-resolution metabolomics of stored PLTs with in vivo PLT recoveries and survivals. Both individual analytes and metabolic pathways that correlate with posttransfusion PLT viability were identified.RESULTSCaffeine metabolites were associated with poor PLT recovery; caffeine metabolism was not ongoing in the PLT bag and remained at prestorage levels. Acylcarnitines, particular fatty acid metabolites, and oxidized fatty acids were associated with poor PLT survivals. Of the myriad metabolic changes during PLT storage, these are the first reported metabolic findings to begin distinguishing which changes are of functional importance regarding posttransfusion PLT performance.CONCLUSIONSTogether, these findings provide novel mechanistic insights into the functional biology of the PLT storage lesion as well as identifying potential targets for modifying donor environment (e.g., caffeine consumption) and also metrics of quality assessment for stored human PLTs.