Phenotypic and functional modulation of bone marrow-derived dendritic cells by porcine reproductive and respiratory syndrome virus
Phenotypic and functional modulation of bone marrow-derived dendritic cells by porcine reproductive and respiratory syndrome virus
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DOI：
10.1016/j.vetmic.2007.12.002
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发表时间：
2008-06-22
影响因子：
3.3
通讯作者：
Chung, Wen-Bin
中科院分区：
文献类型：
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作者：
Chang, Hsueh-Chen;Peng, Yu-Tang;Chung, Wen-Bin
有充分的证据表明，在感染和接种疫苗的猪中，对猪繁殖与呼吸综合征病毒（PRRSV）的有效免疫力的发展存在延迟。这表明，PRRSV可能具有一些固有的特性，以逃避宿主的防御机制，在感染的早期阶段。树突状细胞（Dendritic cells，DC）在T细胞应答病毒抗原的活化和控制中起关键作用。在这项研究中，我们研究了骨髓来源的未成熟DC（BM-imDC）的表型和功能特性的变化，发生感染后的PRRSV结果表明，BM-imDC是允许的PRRSV感染，因为生产性复制发生在这些细胞。在感染后48 h，观察到MHC I分子的表达沿着下调，而CD 80/86的表达上调。同样在PRRSV感染后48 h，注意到BM-imDCs的IL-10分泌显著增加。结果提示，PRRSV感染后BM-imDCs抑制MHC I表达和增强IL-10分泌可能是病毒逃避宿主免疫防御的策略之一。(C)2008由爱思唯尔公司出版
It is well documented that there is a delay in the development of effective immunity to porcine reproductive and respiratory syndrome virus (PRRSV) in infected and vaccinated pigs. This suggests that PRRSV might possess some inherent properties to evade host defense mechanisms during the early stage of infection. Dendritic cells (DCs) play a crucial role in the activation and control of T-cells in response to viral antigens. In this study, we investigated the phenotypic and functional property changes of bone marrow-derived immature DCs (BM-imDCs) that take place after infection by PRRSV Results showed that BM-imDCs were permissive to PRRSV infection, as productive replication took place in these cells. A down-regulated expression of MHC I molecules along with an up-regulated expression of CD80/86 is observed at 48 h following infection. Also at 48 h following PRRSV infection, a significant increase of IL-10 secretion by BM-imDCs was noticed. Results suggest that the inhibited expression of MHC I and the enhanced secretion of IL-10 by BM-imDCs after PRRSV infection might be among the strategies used by the virus to evade the host immune defenses. (C) 2008 Published by Elsevier B.V.