Synthesis and secretion of selenoprotein P by cultured rat astrocytes.
Synthesis and secretion of selenoprotein P by cultured rat astrocytes.
复制标题
培养的大鼠星形胶质细胞硒蛋白 P 的合成和分泌。
DOI：
10.1016/s0304-4165(00)00035-0
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Burk,RF
中科院分区：
文献类型：
--
作者：
Yang,X;Hill,KE;Maguire,MJ;Burk,RF
硒蛋白P是一种细胞外蛋白，被认为具有氧化防御功能。它对培养的神经元具有促进存活的特性，其mRNA存在于脑中。本研究旨在确定大鼠脑硒蛋白P的一级结构，并评估其生产培养的脑细胞。从大鼠脑cDNA文库中分离到硒蛋白P的cDNA，发现其编码与大鼠肝cDNA相同的肽序列。因此，脑硒蛋白P的一级结构与肝硒蛋白P相同。星形胶质细胞和小脑颗粒细胞制备（CGC）从大鼠脑中获得，并建立在文化。估计CGC含有高达5%的神经胶质细胞。两种制剂均含有硒蛋白P mRNA。在孵育期间与75硒标记的亚硒酸盐，两种细胞制剂分泌a75硒标记的蛋白到培养基中，对应的大小硒蛋白P。此外，75硒标记的蛋白质可以沉淀从两种介质与抗血清硒蛋白P。这表明，星形胶质细胞和CGC分泌硒蛋白P。硒蛋白P是在大脑中，可能有氧化防御功能。
Selenoprotein P is an extracellular protein that has been postulated to have an oxidant defense function. It has survival-promoting properties for cultured neurons and its mRNA is present in the brain. This study sought to determine the primary structure of rat brain selenoprotein P and to assess its production by cultured brain cells. The cDNA of selenoprotein P was isolated from a rat brain cDNA library and was found to encode the same peptide sequence as rat liver cDNA. Thus the primary structure of brain selenoprotein P is the same as selenoprotein P from liver. Astrocytes and a cerebellar granule cell preparation (CGC) were obtained from rat brains and established in culture. The CGC was estimated to contain up to 5% glial cells. Both preparations were shown to contain selenoprotein P mRNA. During incubation with75Se-labeled selenite, both cell preparations secreted a75Se-labeled protein into the medium that corresponded in size to selenoprotein P. Also, the75Se-labeled protein could be precipitated from both media with an antiserum to selenoprotein P. This shows that astrocytes and the CGC secrete selenoprotein P. Selenoprotein P is made in the brain and may have an oxidant defense function there.