Expression Analyses of Soybean VOZ Transcription Factors and the Role of GmVOZ1G in Drought and Salt Stress Tolerance
Expression Analyses of Soybean VOZ Transcription Factors and the Role of GmVOZ1G in Drought and Salt Stress Tolerance
复制标题
大豆 VOZ 转录因子的表达分析及 GmVOZ1G 在耐旱和耐盐胁迫中的作用
DOI：
10.3390/ijms21062177
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发表时间：
2020-03-01
影响因子：
5.6
通讯作者：
Ma, You-Zhi
中科院分区：
文献类型：
--
作者：
Li, Bo;Zheng, Jia-Cheng;Ma, You-Zhi
维管束植物单锌指（VOZ）转录因子是一种植物特异性单锌指型转录激活剂，参与调节许多生物过程，例如花诱导和发育、防御病原体以及对多种类型的非生物胁迫的反应。据报道，大豆 (Glycine max) 基因组中存在 6 个 VOZ 转录因子编码基因 (GmVOZ)。尽管如此，目前有关 GmVOZ 的信息还很少。在本研究中，GmVOZ 被克隆并表征。 GmVOZ 基因编码在酵母细胞中具有转录激活活性的蛋白质。 GmVOZ1E、GmVOZ2B 和 GmVOZ2D 基因产物广泛分散在细胞质中，而 GmVOZ1G 主要位于细胞核中。 GmVOZ 在脱水、盐和水杨酸 (SA) 胁迫条件下表现出差异表达谱。其中，GmVOZ1G 在所有应激处理下均表现出显着诱导表达。大豆毛状根中 GmVOZ1G 的过度表达导致对干旱和盐胁迫的更强耐受性。相比之下，抑制 GmVOZ1G 的 RNA 干扰 (RNAi) 大豆毛状根对这两种胁迫更敏感。在干旱处理下，毛状根过度表达的大豆复合植株具有较高的相对含水量（RWC）。为了应对干旱和盐胁迫，在毛状根过度表达的大豆复合幼苗中观察到较低的丙二醛（MDA）积累和较高的过氧化物酶（POD）和超氧化物歧化酶（SOD）活性。在 RNAi 系中获得了每个生理参数的相反结果。总之，GmVOZ1G 正向调节大豆毛状根的干旱和盐胁迫耐受性。我们的结果对于非生物胁迫下大豆 VOZ 转录因子的功能表征具有重要价值。
Vascular plant one-zinc-finger (VOZ) transcription factor, a plant specific one-zinc-finger-type transcriptional activator, is involved in regulating numerous biological processes such as floral induction and development, defense against pathogens, and response to multiple types of abiotic stress. Six VOZ transcription factor-encoding genes (GmVOZs) have been reported to exist in the soybean (Glycine max) genome. In spite of this, little information is currently available regarding GmVOZs. In this study, GmVOZs were cloned and characterized. GmVOZ genes encode proteins possessing transcriptional activation activity in yeast cells. GmVOZ1E, GmVOZ2B, and GmVOZ2D gene products were widely dispersed in the cytosol, while GmVOZ1G was primarily located in the nucleus. GmVOZs displayed a differential expression profile under dehydration, salt, and salicylic acid (SA) stress conditions. Among them, GmVOZ1G showed a significantly induced expression in response to all stress treatments. Overexpression of GmVOZ1G in soybean hairy roots resulted in a greater tolerance to drought and salt stress. In contrast, RNA interference (RNAi) soybean hairy roots suppressing GmVOZ1G were more sensitive to both of these stresses. Under drought treatment, soybean composite plants with an overexpression of hairy roots had higher relative water content (RWC). In response to drought and salt stress, lower malondialdehyde (MDA) accumulation and higher peroxidase (POD) and superoxide dismutase (SOD) activities were observed in soybean composite seedlings with an overexpression of hairy roots. The opposite results for each physiological parameter were obtained in RNAi lines. In conclusion, GmVOZ1G positively regulates drought and salt stress tolerance in soybean hairy roots. Our results will be valuable for the functional characterization of soybean VOZ transcription factors under abiotic stress.